Activation of Human Osteoblasts via Different Bovine Bone Substitute Materials With and Without Injectable Platelet Rich Fibrin in vitro

Solomiya Kyyak; Sebastian Blatt; Eik Schiegnitz; Diana Heimes; Henning Staedt; Daniel G E Thiem; Keyvan Sagheb; Bilal Al-Nawas; Peer W Kämmerer

doi:10.3389/fbioe.2021.599224

Activation of Human Osteoblasts via Different Bovine Bone Substitute Materials With and Without Injectable Platelet Rich Fibrin in vitro

Front Bioeng Biotechnol. 2021 Feb 17:9:599224. doi: 10.3389/fbioe.2021.599224. eCollection 2021.

Authors

Solomiya Kyyak¹, Sebastian Blatt¹, Eik Schiegnitz¹, Diana Heimes¹, Henning Staedt^{2

3}, Daniel G E Thiem¹, Keyvan Sagheb¹, Bilal Al-Nawas¹, Peer W Kämmerer¹

Affiliations

¹ Department of Oral- and Maxillofacial Surgery, University Medical Center Mainz, Mainz, Germany.
² Private Practice, University Medical Center Rostock, Rostock, Germany.
³ Department of Prosthodontics and Materials Science, University Medical Center Rostock, Rostock, Germany.

Abstract

Introduction: The aim of the in vitro study was to compare the effect of four bovine bone substitute materials (XBSM) with and without injectable platelet-reach fibrin for viability and metabolic activity of human osteoblasts (HOB) as well as expression of alkaline phosphatase (ALP), bone morphogenetic protein 2 (BMP-2), and osteonectin (OCN).

Materials and methods: Cerabone^® (CB), Bio-Oss^® (BO), Creos Xenogain^® (CX) and MinerOss^® X (MO) ± i-PRF were incubated with HOB. At day 3, 7, and 10, cell viability and metabolic activity as well as expression of ALP, OCN, and BMP-2, was examined.

Results: For non-i-PRF groups, the highest values concerning viability were seen for CB at all time points. Pre-treatment with i-PRF increased viability in all groups with the highest values for CB-i-PRF after 3 and 7 and for CX-i-PRF after 10 days. For metabolic activity, the highest rate among non-i-PRF groups was seen for MO at day 3 and for CB at day 7 and 10. Here, i-PRF groups showed higher values than non-i-PRF groups (highest values: CB + i-PRF) at all time points. There was no difference in ALP-expression between groups. For OCN expression in non-i-PRF groups, CB showed the highest values after day 3, CX after day 7 and 10. Among i-PRF-groups, the highest values were seen for CX + i-PRF. At day 3, the highest BMP-2 expression was observed for CX. Here, for i-PRF groups, the highest increase was seen for CX + i-PRF at day 3. At day 7 and 10, there was no significant difference among groups.

Conclusion: XBSM sintered under high temperature showed increased HOB viability and metabolic activity through the whole period when compared to XBSM manufactured at lower temperatures. Overall, the combination of XBSM with i-PRF improved all cellular parameters, ALP and BMP-2 expression at earlier stages as well as OCN expression at later stages.

Keywords: PCR; bone substitute; bovine bone; in vitro; osteoblast; platelet rich fibrin (PRF); proliferation; vitality.