Biocompatibility and bioactive potential of the NeoMTA Plus endodontic bioceramic-based sealer

Roberto Alameda Hoshino; Mateus Machado Delfino; Guilherme Ferreira da Silva; Juliane Maria Guerreiro-Tanomaru; Mário Tanomaru-Filho; Estela Sasso-Cerri; Paulo Sérgio Cerri

doi:10.5395/rde.2021.46.e4

Biocompatibility and bioactive potential of the NeoMTA Plus endodontic bioceramic-based sealer

Restor Dent Endod. 2020 Dec 17;46(1):e4. doi: 10.5395/rde.2021.46.e4. eCollection 2021 Feb.

Authors

Roberto Alameda Hoshino¹, Mateus Machado Delfino¹, Guilherme Ferreira da Silva², Juliane Maria Guerreiro-Tanomaru¹, Mário Tanomaru-Filho¹, Estela Sasso-Cerri³, Paulo Sérgio Cerri³

Affiliations

¹ Department of Restorative Dentistry, Dental School, São Paulo State University (UNESP), Araraquara, SP, Brazil.
² Pro-Rectory of Research and Post-graduation, School of Dentistry, Universidade Sagrado Coração (USC), Bauru, SP, Brazil.
³ Department of Morphology, Genetics, Orthodontics and Pediatric Dentistry, Laboratory of Histology and Embryology, Dental School, São Paulo State University (UNESP), Araraquara, SP, Brazil.

Abstract

Objectives: This study evaluated the biocompatibility and bioactive potential of NeoMTA Plus mixed as a root canal sealer in comparison with MTA Fillapex.

Materials and methods: Polyethylene tubes filled with NeoMTA Plus (n = 20), MTA Fillapex (n = 20), or nothing (control group, CG; n = 20) were inserted into the connective tissue in the dorsal subcutaneous layer of rats. After 7, 15, 30 and 60 days, the specimens were processed for paraffin embedding. The capsule thickness, collagen content, and number of inflammatory cells (ICs) and interleukin-6 (IL-6) immunolabeled cells were measured. von Kossa-positive structures were evaluated and unstained sections were analyzed under polarized light. Two-way analysis of variance was performed, followed by the post hoc Tukey test (p ≤ 0.05).

Results: At 7 days, the capsules around NeoMTA Plus and MTA Fillapex had more ICs and IL-6-immunostained cells than the CG. However, at 60 days, there was no significant difference in the IC number between NeoMTA Plus and the CG (p = 0.1137) or the MTA Fillapex group (p = 0.4062), although a greater number of IL-6-immunostained cells was observed in the MTA Fillapex group (p = 0.0353). From 7 to 60 days, the capsule thickness of the NeoMTA Plus and MTA Fillapex specimens significantly decreased, concomitantly with an increase in the collagen content. The capsules around root canal sealers showed positivity to the von Kossa stain and birefringent structures.

Conclusions: The NeoMTA Plus root canal sealer is biocompatible and exhibits bioactive potential.

Keywords: Bioactive potential; Biocompatibility; Immunohistochemistry; Inflammatory reaction; Interleukin-6.