Evaluation of the Baermann-Wetzel method for detecting lungworm larvae in wild ruminants from faecal samples

J Helminthol. 2021 Mar 8:95:e13. doi: 10.1017/S0022149X21000067.

Abstract

Lungworms can exert a negative impact on wild ruminant fitness; for this reason, the diagnosis of the associated diseases is an important prevention measure. The Baerman-Wetzel technique is the most usual method for the diagnosis of bronchopulmonary nematodes and is based on the active migration and movement of their first-stage larvae. Pulmonary tissue samples are frequently used for the post-mortem diagnosis of these parasites, but this kind of sample is not always available and easy to obtain. Faecal samples represent a more accessible choice for parasite monitoring. This work aimed to evaluate the agreement between the results obtained by the Baermann-Wetzel technique when samples of lung parenchyma or faeces from wild ruminants are used. A good level of agreement as well as a similar sensitivity between the two types of sample were observed, validating the use of faecal samples as a less invasive and cost-effective alternative for the monitoring of lungworm in wild ruminant populations.

Keywords: Baermann–Wetzel technique; Bland–Altmann; diagnostic; lungworms; wild ruminant.

MeSH terms

  • Animals
  • Animals, Wild / parasitology
  • Environmental Monitoring
  • Feces / parasitology
  • Larva
  • Lung / parasitology
  • Nematoda* / isolation & purification
  • Ruminants / parasitology*
  • Strongylida Infections* / veterinary