Characterization of human induced pluripotent stem cells line JLUEYEi002-A from a 48 year old healthy male

Hong Wu; Yan Cheng; Zhenshan Lv; Lidi Liu; Weiquan Gong; Shaokun Zhang

doi:10.1016/j.scr.2021.102221

Characterization of human induced pluripotent stem cells line JLUEYEi002-A from a 48 year old healthy male

Stem Cell Res. 2021 May:53:102221. doi: 10.1016/j.scr.2021.102221. Epub 2021 Feb 26.

Authors

Hong Wu¹, Yan Cheng¹, Zhenshan Lv², Lidi Liu², Weiquan Gong², Shaokun Zhang³

Affiliations

¹ Eye Center of 2nd Hospital, Jilin University, People's Republic of China.
² The Department of Spine Surgery, 1st Hospital, Jilin University, Jilin Engineering Research Center for Spine and Spinal Cord Injury, People's Republic of China.
³ The Department of Spine Surgery, 1st Hospital, Jilin University, Jilin Engineering Research Center for Spine and Spinal Cord Injury, People's Republic of China. Electronic address: shaokun@jlu.edu.cn.

PMID: 33677395
DOI: 10.1016/j.scr.2021.102221

Abstract

Peripheral blood was extracted from a 48-year old healthy male donor. Induced pluripotent stem cells (iPSC) were reprogrammed by sendai virus encoding Klf-4, c-Myc, Oct-4, and Sox-2. The iPSC line showed pluripotency, which was verified by immunofluorescence staining. The iPSC line showed normal karyotype, and could form embryoid bodies in vitro and differentiate into the 3 germ layers in vivo. This cell line can be served as healthy control for studying inherited disease.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Cell Differentiation
Cellular Reprogramming
Humans
Induced Pluripotent Stem Cells*
Karyotype
Leukocytes, Mononuclear
Male
Middle Aged