Determination of maytansinoids in Trewia nudiflora using QuEChERS extraction combined with HPLC

Ya-Nan Li; Jia-Nan Li; Qin Ouyang; Yu-Bo Zhou; Chun Lei; Ming-Jun Cui; Kai-Cong Fu; Jia Li; Jian-Ming Huang; Ai-Jun Hou

doi:10.1016/j.jpba.2021.113993

Determination of maytansinoids in Trewia nudiflora using QuEChERS extraction combined with HPLC

J Pharm Biomed Anal. 2021 May 10:198:113993. doi: 10.1016/j.jpba.2021.113993. Epub 2021 Feb 26.

Authors

Ya-Nan Li¹, Jia-Nan Li², Qin Ouyang¹, Yu-Bo Zhou³, Chun Lei¹, Ming-Jun Cui⁴, Kai-Cong Fu⁴, Jia Li⁵, Jian-Ming Huang⁶, Ai-Jun Hou⁷

Affiliations

¹ School of Pharmacy, Fudan University, Shanghai 201203, China.
² Open Studio for Druggability Research of Marine Natural Products, Pilot National Laboratory for Marine Science and Technology (Qingdao), Qingdao 266237, China; National Center for Drug Screening, State Key Laboratory of Drug Research, Shanghai Institute of Materia Medica, Chinese Academy of Sciences, Shanghai 201203, China; University of Chinese Academy of Sciences, Beijing 100049, China; School of Chinese Materia Medica, Nanjing University of Chinese Medicine, Nanjing 210023, China.
³ Open Studio for Druggability Research of Marine Natural Products, Pilot National Laboratory for Marine Science and Technology (Qingdao), Qingdao 266237, China; National Center for Drug Screening, State Key Laboratory of Drug Research, Shanghai Institute of Materia Medica, Chinese Academy of Sciences, Shanghai 201203, China; University of Chinese Academy of Sciences, Beijing 100049, China; School of Chinese Materia Medica, Nanjing University of Chinese Medicine, Nanjing 210023, China. Electronic address: ybzhou@simm.ac.cn.
⁴ Puer Institute of Traditional Ethnomedicine, Yunnan 665000, China.
⁵ Open Studio for Druggability Research of Marine Natural Products, Pilot National Laboratory for Marine Science and Technology (Qingdao), Qingdao 266237, China; National Center for Drug Screening, State Key Laboratory of Drug Research, Shanghai Institute of Materia Medica, Chinese Academy of Sciences, Shanghai 201203, China; University of Chinese Academy of Sciences, Beijing 100049, China; School of Chinese Materia Medica, Nanjing University of Chinese Medicine, Nanjing 210023, China. Electronic address: jli@simm.ac.cn.
⁶ School of Pharmacy, Fudan University, Shanghai 201203, China. Electronic address: jmhuang@shmu.edu.cn.
⁷ School of Pharmacy, Fudan University, Shanghai 201203, China. Electronic address: ajhou@shmu.edu.cn.

PMID: 33677280
DOI: 10.1016/j.jpba.2021.113993

Abstract

Three maytansinoids with strong cytotoxicities, dehydrotrewiasine, maytanbutine, and trewiasine, were isolated and identified from Trewia nudiflora, and maytanbutine was obtained from this plant for the first time. A quick, easy, cheap, effective, rugged, and safe (QuEChERS) extraction combined with high-performance liquid chromatography (HPLC) was established to determine the three maytansinoids in T. nudiflora. The effects of major factors on the extraction efficiency of the QuEChERS method were evaluated and the optimal conditions using acetonitrile-ethyl acetate (1:1, v/v) as the extraction solvent and PestiCarb as the clean-up sorbents were established. Compared with Soxhlet extraction (SE) and ultrasonic-assisted extraction (UAE), the QuEChERS method was easy-to-operate and afforded a cleaner extract. A phenomenex HyperClone BDS C₁₈ column was used for HPLC analysis. Methanol-acetonitrile-water was chosen as mobile phase for gradient elution. Method validation showed that all analytes showed good linearity (r > 0.999) over the investigated ranges and satisfactory recoveries ranging from 95.0% to 105.0%. The developed QuEChERS-HPLC method was simple, efficient, and applicable to the determination of maytansinoids in T. nudiflora.

Keywords: HPLC; Maytansinoids; QuEChERS method; Trewia nudiflora.

MeSH terms

Chromatography, High Pressure Liquid
Solid Phase Extraction
Solvents
Tandem Mass Spectrometry*
Water*

Substances

Solvents
Water