Overexpression of isochorismate synthase enhances salt tolerance in barley

Abstract

Isochorismate synthase (ICS) is a key enzyme for the synthesis of salicylic acid (SA) in plants. SA plays an important role in the response of plants to abiotic stress. In this study, transgenic barley was constructed to evaluate the function of ICS under salt stress. ICS_OE lines showed obvious salt stress tolerance, this results from the increased outward Na⁺ flux and inward K⁺ flux in roots, thereby maintaining a lower cytosolic Na⁺/K⁺ ratio under salt stress. Overexprssion of ICS also improved Na⁺ sequestration in shoots under salt stress. In addition, ICS_OE lines displayed less accumulation of reactive oxygen species and oxidative damage, accompanied by higher activity of antioxidant enzymes. The improved Na⁺/K⁺ ratio, Na⁺ sequestration, and antioxidative competence play an important role in the enhanced salt tolerance of ICS_OE lines. These findings help to elucidate the abiotic stress resistance of the ICS pathway in barley.

Keywords: Antioxidative competence; Barely; Isochorismate synthase; Na(+)/K(+) ratio; Salt tolerance.