Zn-doped MnO2 nanocoating with enhanced catalase-mimetic activity and cytocompatibility protects pre-osteoblasts against H2O2-induced oxidative stress

Shiwei Liu; Kai Li; Tao Hu; Dandan Shao; Shansong Huang; Youtao Xie; Xuebin Zheng

doi:10.1016/j.colsurfb.2021.111666

Zn-doped MnO₂ nanocoating with enhanced catalase-mimetic activity and cytocompatibility protects pre-osteoblasts against H₂O₂-induced oxidative stress

Colloids Surf B Biointerfaces. 2021 Jun:202:111666. doi: 10.1016/j.colsurfb.2021.111666. Epub 2021 Mar 1.

Authors

Shiwei Liu¹, Kai Li², Tao Hu³, Dandan Shao¹, Shansong Huang⁴, Youtao Xie¹, Xuebin Zheng⁵

Affiliations

¹ Key Laboratory of Inorganic Coating Materials CAS, Shanghai Institute of Ceramics, Chinese Academy of Sciences, Shanghai, China; Center of Materials Science and Optoelectronics Engineering, University of Chinese Academy of Sciences, Beijing, China.
² Key Laboratory of Inorganic Coating Materials CAS, Shanghai Institute of Ceramics, Chinese Academy of Sciences, Shanghai, China; Center of Materials Science and Optoelectronics Engineering, University of Chinese Academy of Sciences, Beijing, China. Electronic address: likai@mail.sic.ac.cn.
³ Department of Spine Surgery, Shanghai East Hospital, Tongji University School of Medicine, Shanghai, China.
⁴ Key Laboratory of Inorganic Coating Materials CAS, Shanghai Institute of Ceramics, Chinese Academy of Sciences, Shanghai, China.
⁵ Key Laboratory of Inorganic Coating Materials CAS, Shanghai Institute of Ceramics, Chinese Academy of Sciences, Shanghai, China; Center of Materials Science and Optoelectronics Engineering, University of Chinese Academy of Sciences, Beijing, China. Electronic address: xbzheng@mail.sic.ac.cn.

PMID: 33677135
DOI: 10.1016/j.colsurfb.2021.111666

Abstract

Therapeutic application in prevention and treatment of bone diseases, particularly osteoporosis, has recently started to emerge for manganese dioxide (MnO₂) nanoparticles and nanocoatings whereby their antioxidant catalase-mimetic property can be exploited to control oxidative stress by reducing the amount of H₂O₂. Doping is an efficient method to enhance the catalase-mimetic activity of MnO₂, which can potentially ameliorate osteogenesis under oxidative stress. Herein, Zn²⁺ doped MnO₂ (Zn-MnO₂) nanocoating was fabricated on orthopedic titanium implant by a facile UV-photolysis reaction. The Zn-MnO₂ nanocoating showed better cytocompatibility than the MnO₂ nanocoating, as indicated by enhanced cell proliferation, differentiation and mineralization of MC3T3-E1 pre-osteoblasts. This was probably due to the increased surface hydrophilicity as well as the combination effect of released Zn²⁺ and Mn²⁺ from the Zn-MnO₂ nanocoating. Importantly, the Zn-MnO₂ nanocoating with enhanced catalase-like activity exerted greater effects to suppress the intracellular oxidation products generation and prevent the depletion of dismutase superoxide levels under H₂O₂-induced oxidative stress, which in turn protected MC3T3-E1 pre-osteoblast functions. Overall, surface modification of titanium implants with the Zn-MnO₂ nanocoating could be utilized to ameliorate oxidative stress-inhibited osteogenesis.

Keywords: Catalase-mimetic activity; Cytocompatibility; Oxidative stress; Pre-osteoblasts; Zn-MnO(2).

MeSH terms

Antioxidants*
Biocompatible Materials
Catalase
Hydrogen Peroxide*
Manganese Compounds
Nanostructures
Osteoblasts
Oxidative Stress
Oxides / pharmacology
Zinc

Substances

Antioxidants
Biocompatible Materials
Manganese Compounds
Oxides
manganese oxide
Hydrogen Peroxide
Catalase
Zinc