The Effect of Selenium Nanoparticles on the Osteogenic Differentiation of MC3T3-E1 Cells

Sang-Cheol Lee; Na-Hyun Lee; Kapil D Patel; Tae-Su Jang; Jonathan Campbell Knowles; Hae-Won Kim; Hae-Hyoung Lee; Jung-Hwan Lee

doi:10.3390/nano11020557

The Effect of Selenium Nanoparticles on the Osteogenic Differentiation of MC3T3-E1 Cells

Nanomaterials (Basel). 2021 Feb 23;11(2):557. doi: 10.3390/nano11020557.

Authors

Sang-Cheol Lee^{1

2}, Na-Hyun Lee^{1

3}, Kapil D Patel^{1

4}, Tae-Su Jang⁵, Jonathan Campbell Knowles^{1

6

7}, Hae-Won Kim^{1

2

3

8

9}, Hae-Hyoung Lee^{1

2

3}, Jung-Hwan Lee^{1

2

3

8

9}

Affiliations

¹ Institute of Tissue Regeneration Engineering (ITREN), Dankook University, 119 Dandae-ro, Cheonan, Chungcheongnam-do 31116, Korea.
² Department of Biomaterials Science, College of Dentistry, Dankook University, 119 Dandae-ro, Cheonan, Chungcheongnam-do 31116, Korea.
³ Department of Nanobiomedical Science & BK21 PLUS NBM Global Research Center for Regenerative Medicine, Dankook University, 119 Dandae-ro, Cheonan, Chungcheongnam-do 31116, Korea.
⁴ Department of Materials Science and Engineering, Korea University, Seoul 02841, Korea.
⁵ Department of Pre-medi, College of Medicine, Dankook University, 119 Dandae-ro, Cheonan, Chungcheongnam-do 31116, Korea.
⁶ UCL Eastman-Korea Dental Medicine Innovation Centre, Dankook University, 119 Dandae-ro, Cheonan, Chungcheongnam-do 31116, Korea.
⁷ Division of Biomaterials and Tissue Engineering, Eastman Dental Institute, University College London, London WC1E 6HH, UK.
⁸ Cell & Matter Institute, Dankook University, Cheonan, Chungcheongnam-do 31116, Korea.
⁹ Department of Regenerative Dental Medicine, College of Dentistry, Dankook University, Cheonan, Chungcheongnam-do 31116, Korea.

Abstract

Reactive oxygen species (ROS) regulate various functions of cells, including cell death, viability, and differentiation, and nanoparticles influence ROS depending on their size and shape. Selenium is known to regulate various physiological functions, such as cell differentiations and anti-inflammatory functions, and plays an important role in the regulation of ROS as an antioxidant. This study aims to investigate the effect of selenium nanoparticles (SeNPs) on the differentiation of osteogenic MC3T3-E1 cells. After fabrication of SeNPs with a size of 25.3 ± 2.6 nm, and confirmation of its oxidase-like activity, SeNPs were added to MC3T3-E1 cells with or without H₂O₂: 5~20 μg/mL SeNPs recovered cells damaged by 200 μM H₂O₂ via the intracellular ROS downregulating role of SeNPs, revealed by the ROS staining assay. The increase in osteogenic maturation with SeNPs was gradually investigated by expression of osteogenic genes at 3 and 7 days, Alkaline phosphatase activity staining at 14 days, and Alizarin red S staining at 28 days. Therefore, the role of SeNPs in regulating ROS and their therapeutic effects on the differentiation of MC3T3-E1 cells were determined, leading to possible applications for bone treatment.

Keywords: MC3T3-E1; nanoparticles; osteogenic differentiation; reactive oxygen species; selenium.

Abstract

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