Effects of the Monomeric Components of Poly-hydroxybutyrate-co-hydroxyhexanoate on the Growth of Vibrio penaeicida In Vitro and on the Survival of Infected Kuruma Shrimp (Marsupenaeus japonicus)

Kimio Fukami; Fumika Takagi; Kohei Sonoda; Hiroshi Okamoto; Daisuke Kaneno; Takao Horikawa; Masaki Takita

doi:10.3390/ani11020567

Effects of the Monomeric Components of Poly-hydroxybutyrate-co-hydroxyhexanoate on the Growth of Vibrio penaeicida In Vitro and on the Survival of Infected Kuruma Shrimp (Marsupenaeus japonicus)

Animals (Basel). 2021 Feb 22;11(2):567. doi: 10.3390/ani11020567.

Authors

Kimio Fukami¹, Fumika Takagi¹, Kohei Sonoda², Hiroshi Okamoto², Daisuke Kaneno¹, Takao Horikawa³, Masaki Takita³

Affiliations

¹ Faculty of Agriculture and Marine Science, Kochi University, Nankoku-Shi, Kochi 783-8502, Japan.
² HIGAHIMARU CO., Ltd. Ichikikushikino-Shi, Kagoshima 896-0046, Japan.
³ Kaneka Corporation, Nakanoshima, Osaka 530-8288, Japan.

Abstract

Here, we investigated the inhibitory effects of the biodegradable, water-insoluble polymer poly-hydroxybutyrate-co-hydroxyhexanoate (PHBH) and its two constituent monomers, the hydroxyalkanoic acids 3-hydroxybutyrate (3HB) and 3-hydroxyhexanoate (3HH), on the growth of the shrimp-pathogenic bacterium Vibrio penaeicida. In vitro experiments revealed that 3HH showed greater growth inhibitory activity than 3HB against V. penaeicida. In addition, the activities of hydroxyalkanoic acids were pH dependent, being greater at pH 6.0 than at pH 7.0. Investigation of the pH of the shrimp gut revealed a pH range of 5.9-6.7 (mean 6.29 ± SD 0.20), indicating that the physiological environment was suitable for 3HB and 3HH to exert their inhibitory activities against V. penaeicida. In vivo bacterial challenge experiments revealed that survival rates in kuruma shrimp (Marsupenaeus japonicus) infected by V. penaeicida were significantly increased in shrimp reared on feed containing PHBH (0.1%-5% w/w PHBH) compared with that in shrimp reared on standard diet alone. Supplementation with PHBH had no significant effects on three shrimp growth parameters: increase in body weight, daily feeding rate, and feed conversion ratio. These results suggest that supplementation of standard diet with PHBH will increase shrimp resistance to infection by V. penaeicida, thereby increasing shrimp aquaculture productivity.

Keywords: Marsupenaeus japonicus; inhibitory effect; pathogenic Vibrio; poly-hydroxybutyrate-co-hydroxyhexanoate (PHBH); short- and medium-chain hydroxyalkanoic acids; shrimp survival.