Challenges in Studying Stem Cell Metabolism

Cesar A Perez-Ramirez; Heather R Christofk

doi:10.1016/j.stem.2021.02.016

Challenges in Studying Stem Cell Metabolism

Cell Stem Cell. 2021 Mar 4;28(3):409-423. doi: 10.1016/j.stem.2021.02.016.

Authors

Cesar A Perez-Ramirez¹, Heather R Christofk²

Affiliations

¹ Department of Biological Chemistry, David Geffen School of Medicine, University of California, Los Angeles (UCLA), Los Angeles, CA 90095, USA.
² Department of Biological Chemistry, David Geffen School of Medicine, University of California, Los Angeles (UCLA), Los Angeles, CA 90095, USA; Jonsson Comprehensive Cancer Center, UCLA, Los Angeles, CA 90095, USA; Eli and Edythe Broad Center of Regenerative Medicine and Stem Cell Research, UCLA, Los Angeles, CA 90095, USA. Electronic address: hchristofk@mednet.ucla.edu.

PMID: 33667361
DOI: 10.1016/j.stem.2021.02.016

Abstract

The expanding field of stem cell metabolism has been supported by technical advances in metabolite profiling and novel functional analyses. While use of these methodologies has been fruitful, many challenges are posed by the intricacies of culturing stem cells in vitro, along with the distinctive scarcity of adult tissue stem cells and the complexities of their niches in vivo. This review provides an examination of the methodologies used to characterize stem cell metabolism, highlighting their utility while placing a sharper focus on their limitations and hurdles the field needs to overcome for the optimal study of stem cell metabolic networks.

Challenges in Studying Stem Cell Metabolism

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Abstract

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