Lipidomics data showing the effect of lipofermata on myeloid-derived suppressor cells in the spleens of tumor-bearing mice

Adeleye Oluwatosin Adeshakin; Funmilayo O Adeshakin; Wan Liu; Hua Li; Dehong Yan; Xiaochun Wan

doi:10.1016/j.dib.2021.106882

Lipidomics data showing the effect of lipofermata on myeloid-derived suppressor cells in the spleens of tumor-bearing mice

Data Brief. 2021 Feb 13:35:106882. doi: 10.1016/j.dib.2021.106882. eCollection 2021 Apr.

Authors

Adeleye Oluwatosin Adeshakin^{1

2}, Funmilayo O Adeshakin^{1

2}, Wan Liu¹, Hua Li³, Dehong Yan^{1

2}, Xiaochun Wan^{1

2}

Affiliations

¹ Guangdong Immune Cell therapy Engineering and Technology Research Center, Center for Protein and Cell-based Drugs, Institute of Biomedicine and Biotechnology, Shenzhen Institutes of Advanced Technology, Chinese Academy of Sciences, Shenzhen, 518055, China.
² University of Chinese Academy of Sciences, Beijing, 100864, China.
³ SUSTech Core Research Facilities, Southern University of Science and Technology, Shenzhen, 518055 China.

Abstract

The regulation of myeloid-derived suppressor cells (MDSCs) function is key for effective tumor immunotherapy. Recent lipidomics data revealed that MDSCs accumulate lipid species thereby promote their immunosuppressive activity on T cells. However, genetic manipulation of fatty acid transport protein 2 in mice reduced lipid accumulation in polymorphonuclear MDSCs. Herein we present for the first time lipidome of splenic MDSCs from B16F10 melanoma-bearing mice treated with FATP2 inhibitor - lipofermata compared to the control group. B16F10 were subcutaneously injected into the left flank of wild-type C57BL/6 mice, either lipofermata or vehicle was administered to the mice every day starting from day 7 post-tumor injection for 2 weeks. CD11b⁺Gr1⁺ cells from the spleen referred to as MDSCs were sorted on a flow cytometer machine for lipid extraction. Lipid was extracted using methyl‑tert‑butyl ether as previously described with slight modification, followed by liquid chromatography-mass spectrophotometry lipid profiling using a Q-Exactive instrument coupled with HPLC. The raw scans were identified and quantified with LipidSearch while raw data for various lipid species available on the Mendeley Data repository [1]. The lipid profiles reveal change in lipid species following blockade of FATP2 expression in MDSCs compared to the control. These data were collected in connection to a co-submitted paper [2].

Keywords: Fatty acid transport protein 2 (FATP2); LC-MS; Lipids; MDSCs; Tumor immunotherapy.