Generation of integration-free induced pluripotent stem cell lines from four pediatric ADHD patients

Leoni Grossmann; Cristine Marie Yde Ohki; Christian Döring; Per Hoffmann; Stefan Herms; Anna Maria Werling; Susanne Walitza; Edna Grünblatt

doi:10.1016/j.scr.2021.102268

Generation of integration-free induced pluripotent stem cell lines from four pediatric ADHD patients

Stem Cell Res. 2021 May:53:102268. doi: 10.1016/j.scr.2021.102268. Epub 2021 Feb 24.

Authors

Leoni Grossmann¹, Cristine Marie Yde Ohki¹, Christian Döring¹, Per Hoffmann², Stefan Herms², Anna Maria Werling¹, Susanne Walitza³, Edna Grünblatt⁴

Affiliations

¹ Department of Child and Adolescent Psychiatry and Psychotherapy, Psychiatric University Hospital Zurich, University of Zurich, Zurich, Switzerland.
² Human Genomics Research Group, Department of Biomedicine, University of Basel, 4031 Basel, Switzerland; Institute of Human Genetics, University of Bonn, School of Medicine & University Hospital Bonn, 53105 Bonn, Germany.
³ Department of Child and Adolescent Psychiatry and Psychotherapy, Psychiatric University Hospital Zurich, University of Zurich, Zurich, Switzerland; Neuroscience Center Zurich, University of Zurich and the ETH Zurich, Zurich, Switzerland; Zurich Center for Integrative Human Physiology, University of Zurich, Switzerland.
⁴ Department of Child and Adolescent Psychiatry and Psychotherapy, Psychiatric University Hospital Zurich, University of Zurich, Zurich, Switzerland; Neuroscience Center Zurich, University of Zurich and the ETH Zurich, Zurich, Switzerland; Zurich Center for Integrative Human Physiology, University of Zurich, Switzerland. Electronic address: edna.gruenblatt@kjpd.uzh.ch.

PMID: 33662736
DOI: 10.1016/j.scr.2021.102268

Abstract

Human induced pluripotent stem cell (iPSC) lines have been derived from four male patients with childhood attention-deficit hyperactivity disorder (ADHD). Children and adolescents between the ages 6 and 18 suffering from ADHD were recruited for this work. Isolated keratinocytes or peripheral blood mononuclear cells from the participants were reprogrammed into iPSCs using non-integrating Sendai virus to deliver the reprogramming factors Oct3/4, Sox2, Klf4 and c-Myc.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Adolescent
Attention Deficit Disorder with Hyperactivity*
Cell Differentiation
Cellular Reprogramming
Child
Humans
Induced Pluripotent Stem Cells*
Keratinocytes
Kruppel-Like Factor 4
Leukocytes, Mononuclear
Male
Sendai virus