CRISPR-Cas12-based nucleic acids detection systems

Ross Ka-Kit Leung; Qiu-Xiang Cheng; Zhi-Le Wu; Gulfaraz Khan; Yang Liu; Hai-Yang Xia; Jin Wang

doi:10.1016/j.ymeth.2021.02.018

CRISPR-Cas12-based nucleic acids detection systems

Methods. 2022 Jul:203:276-281. doi: 10.1016/j.ymeth.2021.02.018. Epub 2021 Mar 2.

Authors

Ross Ka-Kit Leung¹, Qiu-Xiang Cheng², Zhi-Le Wu³, Gulfaraz Khan⁴, Yang Liu⁵, Hai-Yang Xia⁶, Jin Wang⁷

Affiliations

¹ Dongguan Maternal and Child Health Care Hospital/Dongguan Institute of Reproductive and Genetic Research, Dongguan 523120, China; Stanley Ho Centre for Emerging Infectious Diseases, Faculty of Medicine, The Chinese University of Hong Kong, Hong Kong Special Administrative Region.
² Tolo Biotechnology Company Limited, Shanghai 200233, China.
³ College of Life Sciences, Shanghai Normal University, Shanghai 200234, China.
⁴ Department of Medical Microbiology and Immunology, College of Medicine & Health Sciences, United Arab Emirates University, United Arab Emirates.
⁵ Shanghai Institute of Quality Inspection and Technical Research/National Quality Supervision and Inspection Center for Food Products (Shanghai), Shanghai 200233, China.
⁶ Institute of Biopharmaceuticals, Taizhou University, Taizhou 318000, China.
⁷ Department of Clinical Laboratory, Shenzhen Second People's Hospital & Institute of Translational Medicine the First Affiliated Hospital of Shenzhen University Health Science Center, Shenzhen 518035, China. Electronic address: wangj01@hotmail.com.

PMID: 33662563
DOI: 10.1016/j.ymeth.2021.02.018

Abstract

Because of the outstanding contribution in genome editing, CRISPR has undoubtedly become the most popular technology around the world and two pioneers are awarded the Nobel Prize in Chemistry this year. Besides, along with the discovery of nonspecific trans-cleavage activities of several Cas proteins such as Cas12 and Cas13, many CRISPR-based molecular diagnostic systems have been successfully created, showing advantages in sensitivity, specificity and operation convenience. Among them, systems with Cas12, which targets DNA and trans-cleaves single-stranded DNA probes, are both simple and highly efficient. Here in this review, we mainly focus on the Cas12-based methods and briefly discuss their applications in nucleic acids detection and beyond.

Keywords: CRISPR; CRISPR diagnostics; Cas12; HOLMES; Trans-cleavage.

Publication types

Review
Research Support, Non-U.S. Gov't

MeSH terms

CRISPR-Cas Systems* / genetics
DNA / genetics
DNA, Single-Stranded
Gene Editing* / methods

Substances

DNA, Single-Stranded
DNA