Rhizobium changzhiense sp. nov., isolated from effective nodules of Vicia sativa L. in North China

Junjie Zhang; Shanshan Peng; Mitchell Andrews; Chunzeng Liu; Yimin Shang; Shuo Li; Entao Wang; Xiaoxia Zhang

doi:10.1099/ijsem.0.004724

Rhizobium changzhiense sp. nov., isolated from effective nodules of Vicia sativa L. in North China

Int J Syst Evol Microbiol. 2019 Jun;71(3). doi: 10.1099/ijsem.0.004724. Epub 2021 Mar 4.

Authors

Junjie Zhang^{1

2}, Shanshan Peng¹, Mitchell Andrews³, Chunzeng Liu⁴, Yimin Shang¹, Shuo Li¹, Entao Wang⁵, Xiaoxia Zhang⁶

Affiliations

¹ College of Food and Bioengineering, Zhengzhou University of Light Industry, Zhengzhou, Henan Province, 450000, PR China.
² Collaborative Innovation Center for Food Production and Safety of Henan province, Zhengzhou 450002, Henan Province, PR China.
³ Faculty of Agriculture and Life Sciences, Lincoln University, Lincoln 7647, New Zealand.
⁴ Institute of Plant Nutrition and Resource Environment, Henan Academy of Agricultural Sciences, Zhengzhou 450000, Henan Province, PR China.
⁵ Departamento de Microbiología, Escuela Nacional de Ciencias Biológicas, Instituto Politécnico Nacional, C.P. 11340, Ciudad de México, Mexico.
⁶ Agricultural Cultural Collection of China, Institute of Agricultural Resources and Regional Planning, Chinese Academy of Agricultural Sciences, Beijing 100080, PR China.

PMID: 33661090
DOI: 10.1099/ijsem.0.004724

Abstract

Three fast-growing rhizobial strains isolated from effective nodules of common vetch (Vicia sativa L.) were characterized using a polyphasic approach. All three strains were assigned to the genus Rhizobium on the basis of the results of 16S rRNA gene sequence analysis. Phylogenetic analysis based on concatenated atpD-recA genes separated the strains into a distinct lineage represented by WYCCWR 11279^T, which showed average nucleotide identity values of 95.40 and 93.61 % with the most similar phylogenetic type strains of Rhizobium sophorae CCBAU 03386^T and Rhizobium laguerreae FB T^T, respectively. The digital DNA-DNA hybridization relatedness values between WYCCWR 11279^T and the closest related type strains were less than 70 %. Therefore, a novel rhizobial species is proposed, Rhizobium changzhiense sp. nov., and strain WYCCWR 11279^T (=HAMBI 3709^T=LMG 31534^T) is designated as the type strain for the novel species.

Keywords: Rhizobia; Rhizobium changzhiense; Root nodule; Vicia sativa.