Derived compounds from lignin have been used as substrates for chemical and biological processes for obtainment bioproducts. The ferulic acid is a lignocellulosic biomass whose biotransformation in flavors compounds was described. The objective of this study was the bioconversion of ferulic acid to 4-vinylguaiacol by Klebsiella pneumoniae TD 4.7. The biotransformation of commercial ferulic acid into 4-vinylguaiacol in a semi synthetic liquid medium containing the ferulic acid at an initial concentration of 300 mg L-1 reached 32.4%. The ferulic acid obtained from alkaline hydrolysis of the sugar cane bagasse at 300 mg L-1 allowed the yield of 1.3 mmol L-1 of 4-vinylguaiacol, corresponding to 81.7% of the ferulic acid content. The data indicated that the bacterial strain decarboxylated the ferulic acid to 4-vinylguaiacol and the presence of an active cell associated ferulic acid decarboxylase. The enzyme showed maximum activity at pH 5.5 and 40 °C and was stable at pH range 4.5 to 9.0 and temperature up 20 to 45 °C. According to these biochemical properties and performance to bioconversion of ferulic acid to 4-vinylguaiacol, this enzyme could be viable for application in food industry.
Keywords: 4-vinylguaiacol; Ferulic acid; Ferulic acid decarboxylase; Klebsiella.
© 2021. Sociedade Brasileira de Microbiologia.