The complete chloroplast genome of Bupleurum marginatum var. stenophyllum (H. Wolff) Shan & Yin Li (Apiaceae), a new substitution for Chinese medicinal material, Bupleuri Radix (Chai hu)

Gaixia Zhang; Hui Wang; Jiemei Jiang; Qiuling Wang; Baoli Li; Linchun Shi; Jianhe Wei

doi:10.1080/23802359.2020.1870899

The complete chloroplast genome of Bupleurum marginatum var. stenophyllum (H. Wolff) Shan & Yin Li (Apiaceae), a new substitution for Chinese medicinal material, Bupleuri Radix (Chai hu)

Mitochondrial DNA B Resour. 2021 Feb 9;6(2):441-443. doi: 10.1080/23802359.2020.1870899.

Authors

Gaixia Zhang¹, Hui Wang^{1

2}, Jiemei Jiang¹, Qiuling Wang¹, Baoli Li¹, Linchun Shi¹, Jianhe Wei¹

Affiliations

¹ Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, China.
² Institute of Sericulture, Chengde Medical University, Chengde, China.

Abstract

The root of Bupleurum marginatum var. stenophyllum (H. Wolff) Shan & Yin Li (Apiaceae), a new substitution for the popular Chinese medicinal material, Bupleuri Radix (Chai hu), is not easily distinguishable via traditional methods. The complete chloroplast genome sequence of B. marginatum var. stenophyllum was characterized using next-generation sequencing and the de novo assembly method. The complete genome was 155,576 bp in length and contained two inverted repeat (IR) regions of 26,311 bp, a large single-copy (LSC) region of 85,351 bp, and a small single-copy (SSC) region of 17,603 bp. It encoded 113 unique genes consisting of 79 protein-coding genes (PCGs), 30 transfer RNA genes, and four ribosomal RNA genes. Importantly, three genes (petB, petD and rps16) with small exon, and one trans-splicing gene (rps12) were correctly annotated. The overall GC content of the B. marginatum var. stenophyllum chloroplast genome is 37.7%. The phylogenetic analyses indicated that B. marginatum var. stenophyllum was closely related to B. marginatum. Moreover, many genetic information sites were available for distinguishing B. marginatum var. stenophyllum from the official 'Chai hu' plant sources, B. scorzonerifolium Willd. and B. chinense DC.

Keywords: Bupleurum marginatum var. stenophyllum; complete genome; discrimination; phylogenetic analysis; substitution.

Grants and funding

This work was supported by the National Key R&D Program of China [grant number 2019YFC1604705, 2019YFC1710800], China Agriculture Research System [grant number CARS-21], and Chinese Academy of Medical Science Innovation Fund for Medical Science [grant number 2016-I2M-2-003, 2020-I2M-2-011].