Lightsheet-based flow cytometer for whole blood with the ability for the magnetic retrieval of objects from the blood flow

Roman A Verkhovskii; Anastasiia A Kozlova; Olga A Sindeeva; Ilya O Kozhevnikov; Ekaterina S Prikhozhdenko; Oksana A Mayorova; Oleg V Grishin; Mikhail A Makarkin; Alexey V Ermakov; Arkady S Abdurashitov; Valery V Tuchin; Daniil N Bratashov

doi:10.1364/BOE.413845

Lightsheet-based flow cytometer for whole blood with the ability for the magnetic retrieval of objects from the blood flow

Biomed Opt Express. 2020 Dec 15;12(1):380-394. doi: 10.1364/BOE.413845. eCollection 2021 Jan 1.

Authors

Affiliations

¹ Saratov State University, 83 Astrakhanskaya str., Saratov 410012, Russia.
² Skolkovo Innovation Center, 3 Nobel str., Moscow 121205, Russia.
³ National Research Tomsk State University, 36 Lenin Avenue, Tomsk 634050, Russia.
⁴ Institute of Precision Mechanics and Control of the RAS, 24 Rabochaya str., Saratov 410028, Russia.

Abstract

Detection and extraction of circulating tumor cells and other rare objects in the bloodstream are of great interest for modern diagnostics, but devices that can solve this problem for the whole blood volume of laboratory animals are still rare. Here we have developed SPIM-based lightsheet flow cytometer for the detection of fluorescently-labeled objects in whole blood. The bypass channel between two blood vessels connected with the external flow cell was used to visualize, detect, and magnetically separate fluorescently-labeled objects without hydrodynamic focusing. Carriers for targeted drug delivery were used as model objects to test the device performance. They were injected into the bloodstream of the rat, detected fluorescently, and then captured from the bloodstream by a magnetic separator prior to filtration in organs. Carriers extracted from the whole blood were studied by a number of in vitro methods.