Abstract
Oxepinamides are fungal oxepine-pyrimidinone-ketopiperazine derivatives. In this study, we elucidated the biosynthetic pathway of oxepinamide D in Aspergillus ustus by gene deletion, heterologous expression, feeding experiments, and enzyme assays. We demonstrated that the cytochrome P450 enzymes catalyzed highly specific and stereoselective oxepin ring formation.
Publication types
-
Research Support, Non-U.S. Gov't
MeSH terms
-
Aspergillus / chemistry
-
Aspergillus / metabolism*
-
Biosynthetic Pathways
-
Catalysis
-
Cytochrome P-450 Enzyme System / chemistry*
-
Cytochrome P-450 Enzyme System / metabolism
-
Enzyme Assays
-
Fungi / chemistry*
-
Fungi / metabolism
-
Molecular Structure
-
Oxepins / chemistry*
Substances
-
Oxepins
-
Cytochrome P-450 Enzyme System