Size-selective DNA nanocage-based activatable CRISPR-Cas12a for sensitive and accurate detection of mature microRNA

Xiaoyi Fu; Yao Yin; Meng Zhang; Fangqi Peng; Yuyan Shi; Yan Liu; Yin Tan; Zilong Zhao; Xia Yin; Jie Song; Guoliang Ke; Xiao-Bing Zhang

doi:10.1039/d1cc00178g

Size-selective DNA nanocage-based activatable CRISPR-Cas12a for sensitive and accurate detection of mature microRNA

Chem Commun (Camb). 2021 Apr 4;57(26):3291-3294. doi: 10.1039/d1cc00178g. Epub 2021 Mar 3.

Authors

Xiaoyi Fu¹, Yao Yin, Meng Zhang, Fangqi Peng, Yuyan Shi, Yan Liu, Yin Tan, Zilong Zhao, Xia Yin, Jie Song, Guoliang Ke, Xiao-Bing Zhang

Affiliation

¹ Molecular Science and Biomedicine Laboratory, State Key Laboratory of Chemo/Biosensing and Chemometrics, College of Chemistry and Chemical Engineering, Hunan University, Changsha, Hunan 410082, China. glke@hnu.edu.cn.

PMID: 33656042
DOI: 10.1039/d1cc00178g

Abstract

The sensitive and accurate detection of mature miRNA without the signal interference by pre-miRNAs is highly important. Herein, a size-selective DNA nanocage-based activatable CRISPR/Cas12a system was developed to achieve this goal.

MeSH terms

CRISPR-Cas Systems*
Clustered Regularly Interspaced Short Palindromic Repeats
DNA / chemistry*
DNA / genetics
Humans
MicroRNAs / analysis*
MicroRNAs / blood
MicroRNAs / genetics
Nanostructures / chemistry
Nucleic Acid Hybridization

Substances

MIRN21 microRNA, human
MicroRNAs
DNA