A brief overview on the application and sources of α-amylase and expression hosts properties in order to production of recombinant α-amylase

Biotechnol Appl Biochem. 2022 Apr;69(2):650-659. doi: 10.1002/bab.2140. Epub 2021 Mar 19.

Abstract

By reducing the activation energy, enzymes accelerate the chemical reaction; therefore, they are good alternative for industrial catalysts. Amylase is a suitable enzyme as a catalyst for the chemical decomposition of starch. This enzyme is of great importance, and its production is highly profitable. α-Amylase is among the most important amylases produced naturally by animals, plants, and microorganisms. Still, the α-amylases produced by bacteria have a special place in industry and commerce. Moreover, a large volume of this enzyme can be produced by selecting an appropriate and optimized host to clone and express the α-amylase gene. The present study briefly reviews the structure, application, sources, and hosts used to produce recombinant α-amylase.

Keywords: enzyme; recombinant protein; α-amylase.

Publication types

  • Review

MeSH terms

  • Amylases* / genetics
  • Amylases* / metabolism
  • Animals
  • Bacteria / metabolism
  • Starch / metabolism
  • alpha-Amylases* / metabolism

Substances

  • Starch
  • Amylases
  • alpha-Amylases