Tracking Root Interactions System (TRIS) Experiment and Quality Control

Hassan Massalha; Elisa Korenblum; Orr H Shapiro; Asaph Aharoni

doi:10.21769/BioProtoc.3211

Tracking Root Interactions System (TRIS) Experiment and Quality Control

Bio Protoc. 2019 Apr 20;9(8):e3211. doi: 10.21769/BioProtoc.3211.

Authors

Hassan Massalha¹, Elisa Korenblum¹, Orr H Shapiro², Asaph Aharoni¹

Affiliations

¹ Department of Plant and Environmental Sciences, Weizmann Institute of Science, Rehovot 76100, Israel.
² Department of Food Sciences, Institute for Postharvest and Food Sciences, Agricultural Research Organization, The Volcani Center, Rishon LeZion 7528809, Israel.

Abstract

Soil organisms are diverse taxonomically and functionally. This ecosystem experiences highly complex networks of interactions, but may also present functionally independent entities. Plant roots, a metabolically active hotspot in the soil, take an essential part in shaping the rhizosphere. Tracking the dynamics of root-microbe interactions at high spatial resolution is currently limited due to methodological intricacy. In this study, we developed a novel microfluidics-based device enabling direct imaging of root-bacteria interactions in real time.

Keywords: Arabidopsis thaliana; Bacillus subtilis; Live-imaging microscopy; Microbial community dynamics; Root-bacteria interactions; Tracking root interactions system (TRIS).