Induction and Analysis of Anti-CD40-induced Colitis in Mice

Barbara Joyce-Shaikh; Daniel J Cua; David Bauché

doi:10.21769/BioProtoc.3153

Induction and Analysis of Anti-CD40-induced Colitis in Mice

Bio Protoc. 2019 Feb 5;9(3):e3153. doi: 10.21769/BioProtoc.3153.

Authors

Barbara Joyce-Shaikh¹, Daniel J Cua¹, David Bauché¹

Affiliation

¹ Merck & Co., Inc., MRL, Palo Alto, CA 94304-1104, USA.

Abstract

Colon inflammation or colitis affects more than 1 million people worldwide. Several pre-clinical models, including chemical-induced (i.e., DSS, TNBS) or pathogen-induced (i.e., Citrobacter rodentium) have been used to study mechanisms involved in the development and regulation of colitis. Anti-CD40 induced colitis model has gained acceptance to study the roles of innate immune cells during acute intestinal inflammation. Here we describe a rapid, robust and reproducible protocol to induce and analyze anti-CD40 mediated colitis in mice.

Keywords: Anti-CD40; Colitis; IBD; IL-22; IL-23; Inflammation; Innate immunity.