Differentiation of Mouse Embryonic Stem Cells to Neuronal Cells Using Hanging Droplets and Retinoic Acid

Jeroen Witteveldt; Sara Macias

doi:10.21769/BioProtoc.3417

Differentiation of Mouse Embryonic Stem Cells to Neuronal Cells Using Hanging Droplets and Retinoic Acid

Bio Protoc. 2019 Nov 5;9(21):e3417. doi: 10.21769/BioProtoc.3417.

Authors

Jeroen Witteveldt¹, Sara Macias¹

Affiliation

¹ Institute of Immunology and Infection Research, School of Biological Sciences, University of Edinburgh, Edinburgh, EH9 3FL, UK.

Abstract

Controlled differentiation of embryonic stem cells is an essential tool in stem cell research. In this protocol, we describe a simple differentiation protocol involving the induction of embryoid body formation in mouse embryonic stem cells (mESC) using hanging droplets, followed by differentiation into a neuronal lineage.

Keywords: Differentiation; Embryoid bodies; Embryonic stem cells; Hanging droplets; Neuronal; Retinoic acid.