Adoptive Transfer of Basophils Enriched from Mouse Spleen

Adrian M Piliponsky; Stephen J Galli

doi:10.21769/BioProtoc.3416

Adoptive Transfer of Basophils Enriched from Mouse Spleen

Bio Protoc. 2019 Nov 5;9(21):e3416. doi: 10.21769/BioProtoc.3416.

Authors

Adrian M Piliponsky^{1

2

3}, Stephen J Galli^{4

5

6}

Affiliations

¹ Center for Immunity and Immunotherapies, Seattle Children's Research Institute, Seattle, WA, USA.
² Departments of Pediatrics and.
³ Pathology, University of Washington School of Medicine, Seattle, WA, USA.
⁴ Department of Pathology, Stanford University School of Medicine, Stanford, CA, USA.
⁵ Sean N. Parker Center for Allergy and Asthma Research, Stanford University School of Medicine, CA, USA.
⁶ Department of Microbiology and Immunology, Stanford University School of Medicine, Stanford, CA, USA.

Abstract

CD49b is a member of the integrin family, expressed on basophils, natural killer (NK) cells and a subset CD4⁺ T cells in the spleen. This protocol describes the adoptive transfer of basophil-enriched CD49b⁺ cells obtained from mouse spleens by magnetic enrichment. This protocol can be used to assess the contribution of basophils or basophil-derived mediators to a certain immune response.

Keywords: Adoptive transfer; Basophils; Magnetic enrichment; Mouse; Spleen.