Endocytosis Detection in Magnaporthe oryzae

Muxing Liu; Zhengguang Zhang

doi:10.21769/BioProtoc.3322

Endocytosis Detection in Magnaporthe oryzae

Bio Protoc. 2019 Aug 5;9(15):e3322. doi: 10.21769/BioProtoc.3322.

Authors

Muxing Liu¹, Zhengguang Zhang¹

Affiliation

¹ Department of Plant Pathology, College of Plant Protection, Nanjing Agricultural University, and Key Laboratory of Integrated Management of Crop Diseases and Pests, Ministry of Education, Nanjing, China.

Abstract

Endocytosis is an intracellular trafficking pathway that occurs in nutrient uptake, signal transduction and reconstruction of cell polarity and is conserved in eukaryotic cells. In fungi, endocytosis plays crucial roles in the physiology of hyphal growth and pathogenicity. vidence for endocytosis in filamentous fungi is detected by the membrane-selective dyes FM4-64. Cells of a range of filamentous fungal species readily take up these dyes. However, the method for endocytosis detection has not been well established in Magnaporthe oryzae. Here, we provide a protocol for tracking endocytosis in Magnaporthe oryzae.

Keywords: Endocytosis; FM4-64; Fungi; Hyphal growth; Intracellular trafficking; Magnaporthe oryzae; Pathogenicity.