Morphological Evaluation of Wound Healing Events in the Excisional Wound Healing Model in Rats

Lígia Reis de Moura Estevão; Puebla Cassini-Vieira; Ana Greice Borba Leite; Apolônia Agnes Vilar de Carvalho Bulhões; Lucíola da Silva Barcelos; Joaquim Evêncio-Neto

doi:10.21769/BioProtoc.3285

Morphological Evaluation of Wound Healing Events in the Excisional Wound Healing Model in Rats

Bio Protoc. 2019 Jul 5;9(13):e3285. doi: 10.21769/BioProtoc.3285.

Authors

Lígia Reis de Moura Estevão¹, Puebla Cassini-Vieira², Ana Greice Borba Leite¹, Apolônia Agnes Vilar de Carvalho Bulhões¹, Lucíola da Silva Barcelos³, Joaquim Evêncio-Neto¹

Affiliations

¹ Department of Morphology and Animal Physiology, Universidade Federal Rural de Pernambuco (UFRPE), Recife-PE, Brazil.
² Department of Medicine, Division of Hematology/Oncology Department of Immunology and Microbiology, Cancer Center Rush University Medical Center, Chicago, USA.
³ Department of Physiology and Biophysics (ICB) Universidade Federal de Minas Gerais (UFMG), Belo Horizonte-MG, Brazil.

Abstract

Skin wound healing is a complex process involving different events such as blood coagulation, inflammation, new blood vessels formation, and extracellular matrix deposition. These events can be observed by using histology techniques. However, the lack of the standardization of such parameters impacts on the reproducibility of results. Here, we describe a protocol to perform macroscopic and microscopic analyses of the events that occur during skin wound healing using the experimental model of excisional wounds in rats.

Keywords: Angiogenesis; Collagen deposition; Epithelialization; Histological score; Inflammation; Rat excisional wound.