Error-Free Synthetic DNA by Molecular Dictation

Philipp Knyphausen; Laurens Lindenburg; Florian Hollfelder

doi:10.1016/j.tibtech.2021.02.001

Error-Free Synthetic DNA by Molecular Dictation

Trends Biotechnol. 2021 Sep;39(9):861-865. doi: 10.1016/j.tibtech.2021.02.001. Epub 2021 Feb 27.

Authors

Philipp Knyphausen¹, Laurens Lindenburg¹, Florian Hollfelder²

Affiliations

¹ Department of Biochemistry, University of Cambridge, 80 Tennis Court Road, Cambridge CB2 1GA, UK.
² Department of Biochemistry, University of Cambridge, 80 Tennis Court Road, Cambridge CB2 1GA, UK. Electronic address: fh111@cam.ac.uk.

PMID: 33653603
DOI: 10.1016/j.tibtech.2021.02.001

Abstract

Synthetic DNA is the linchpin of the rapidly accelerating biotechnological era and is perhaps the most promising candidate for long-term digital data storage. Despite huge advances, manufacturing error-free DNA at low cost and high throughput remains challenging. Borrowing from well-established sequencing-by-synthesis technologies, we describe a new solution for DNA error correction.

Keywords: DNA synthesis; error correction; synthetic biology.

Publication types

Research Support, Non-U.S. Gov't
Review

MeSH terms

Biotechnology* / methods
Biotechnology* / trends
DNA* / chemical synthesis
DNA* / genetics
High-Throughput Nucleotide Sequencing
Information Storage and Retrieval* / methods
Information Storage and Retrieval* / trends

Substances

DNA