Relationship between endophytic microbial diversity and grain quality in wheat exposed to multi-generational CO2 elevation

Abstract

To explore the potential association between the diversity of endophytic microorganisms and modifications of grain quality in wheat exposed to multi-generational elevated CO₂ concentration, the grain quality attributes and microbial diversity were tested after five generations successively grown in ambient CO₂ concentration (F5_A, 400 μmol L^-1) and elevated CO₂ concentration (F5_E, 800 μmol L^-1). Elevated CO₂ concentration significantly increased the grain number and starch concentration, while decreased the grain protein concentration. Multi-generational exposure to elevated CO₂ concentration also led to significant changes in grain amino acid concentration. In response to the elevated CO₂ concentration, Pseudomonas, Rhodococcus, Ralstonia, and Klebsiella were the dominant bacterial genera, while Penicillium, Cutaneotrichosporon, Fusarium, Sarocladium, Acremonium and Aspergillus were the dominant fungal genera in wheat grain. A significantly positive correlation was found between Pseudomonas, Penicillium and ratio of starch to protein concentration, implying that the multi-generational CO₂ elevation induced modifications in grain quality might be associated with the changes in grain microbial diversity. The results of this study suggest that the endophytic microbes may play an important role in modulating the grain nutritional quality in wheat under multi-generational e[CO₂] exposure, through regulating starch and N metabolism and production of secondary metabolites.

Keywords: Elevated CO(2); Grain quality; Microbial diversity; Transgenerational effect; Triticum aestivum.