Analysis of Copy Number Variation in Urine: c-Myc Evaluation Using a Real-Time PCR Approach

Methods Mol Biol. 2021:2292:49-56. doi: 10.1007/978-1-0716-1354-2_5.

Abstract

Urine cell-free DNA has been shown as an informative noninvasive source of biomarkers for a number of diseases, especially for urological cancers. Starting from the hypothesis that the gain of c-Myc gene is a frequent aberration in several cancer types, including prostate cancer, we analyzed c-Myc copy number variation in urine, studying a little case series of prostate cancer patients, to test its feasibility. Here we report a general protocol that may be considered to analyze gene copy number variation in the urine cell-free fraction.

Keywords: Copy number; Prostate cancer; Urine; c-Myc.

MeSH terms

  • Biomarkers, Tumor / genetics
  • Biomarkers, Tumor / urine
  • Cell-Free Nucleic Acids / genetics*
  • Cell-Free Nucleic Acids / urine
  • DNA Copy Number Variations*
  • Genes, myc*
  • Humans
  • Male
  • Prostatic Neoplasms / genetics*
  • Prostatic Neoplasms / urine
  • Real-Time Polymerase Chain Reaction / methods*

Substances

  • Biomarkers, Tumor
  • Cell-Free Nucleic Acids