Xylo-oligosaccharide microparticles with synbiotic potential obtained from enzymatic hydrolysis of sugarcane straw

Manoela Martins; Karen Cristina Guedes Silva; Patrícia Félix Ávila; Ana Carla Kawazoe Sato; Rosana Goldbeck

doi:10.1016/j.foodres.2020.109827

Xylo-oligosaccharide microparticles with synbiotic potential obtained from enzymatic hydrolysis of sugarcane straw

Food Res Int. 2021 Feb:140:109827. doi: 10.1016/j.foodres.2020.109827. Epub 2020 Oct 31.

Authors

Manoela Martins¹, Karen Cristina Guedes Silva², Patrícia Félix Ávila¹, Ana Carla Kawazoe Sato², Rosana Goldbeck³

Affiliations

¹ Bioprocess and Metabolic Engineering Laboratory, Department of Food Engineering, University of Campinas, Campinas, SP, Brazil.
² Process Engineering Laboratory, School of Food Engineering, University of Campinas - UNICAMP, Campinas, SP, Brazil.
³ Bioprocess and Metabolic Engineering Laboratory, Department of Food Engineering, University of Campinas, Campinas, SP, Brazil. Electronic address: goldbeck@unicamp.br.

PMID: 33648164
DOI: 10.1016/j.foodres.2020.109827

Abstract

Synbiotic formulations and microencapsulation techniques have been explored in food industries to guarantee the viability of probiotic organisms; playing an important role in microbiota balance. Microparticles of alginate, gelatin and xylo-oligosaccharides (XOS) were produced by external gelation with the purpose of enhancing the survival rate of the probiotic L. acidophilus. XOS was obtained through enzymatic hydrolysis of xylan extracted from sugarcane straw, achieving more than 70% conversion and used for microparticle preparation. Microparticles containing 3% XOS provided greater cell protection during exposure to the gastrointestinal tract and during refrigerated storage; keeping 97.86 ± 0.44% of viability during 28 days of storage and enabling 87.50 ± 0.02% survival after digestive simulation. However, particles without XOS showed 84.49 ± 0.59% of viability after storage and 68.45 ± 0.03% after digestion assay. These results lead to promising applications in synbiotic and functional food formulations comprised of components requiring extended shelf-life, protection from gastrointestinal conditions and gradual bioactive delivery.

Keywords: L. acidophilus; Lignocellulosic biomass; Microencapsulation; Prebiotic.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Hydrolysis
Oligosaccharides
Probiotics*
Saccharum*
Synbiotics*

Substances

Oligosaccharides