TrIPP-a method for tracking the inheritance patterns of proteins in living cells-reveals retention of Tup1p, Fpr4p, and Rpd3L in the mother cell

Morgane Auboiron; Pauline Vasseur; Saphia Tonazzini; Arame Fall; Francesc Rubert Castro; Iva Sučec; Khadija El Koulali; Serge Urbach; Marta Radman-Livaja

doi:10.1016/j.isci.2021.102075

TrIPP-a method for tracking the inheritance patterns of proteins in living cells-reveals retention of Tup1p, Fpr4p, and Rpd3L in the mother cell

iScience. 2021 Jan 20;24(2):102075. doi: 10.1016/j.isci.2021.102075. eCollection 2021 Feb 19.

Authors

Morgane Auboiron^{1

2}, Pauline Vasseur¹, Saphia Tonazzini^{1

2}, Arame Fall^{1

2}, Francesc Rubert Castro¹, Iva Sučec¹, Khadija El Koulali^{2

3}, Serge Urbach^{2

3}, Marta Radman-Livaja^{1

2}

Affiliations

¹ Institut de Génétique Moléculaire de Montpellier, UMR 5535 CNRS, 1919 route de Mende, 34293 Montpellier cedex 5, France.
² Université de Montpellier, 163 rue Auguste Broussonnet, 34090 Montpellier, France.
³ Functional Proteomics Platform, IGF _ CNRS INSERM, Université de Montpellier, 141 rue de la Cardonille, 34094 Montpellier cedex 5, France.

Abstract

Inheritance of chromatin-bound proteins theoretically plays a role in the epigenetic transmission of cellular phenotypes. Protein segregation during cell division is however poorly understood. We now describe TrIPP (Tracking the Inheritance Patterns of Proteins): a live cell imaging method for tracking maternal proteins during asymmetric cell divisions of budding yeast. Our analysis of the partitioning pattern of a test set of 18 chromatin-associated proteins reveals that abundant and moderately abundant maternal proteins segregate stochastically and symmetrically between the two cells with the exception of Rxt3p, Fpr4p, and Tup1p, which are preferentially retained in the mother. Low abundance proteins also tend to be retained in the mother cell with the exception of Sir2p and the linker histone H1. Our analysis of chromatin protein behavior in single cells reveals potentially general trends such as coupled protein synthesis and decay and a correlation between protein half-lives and cell-cycle duration.

Keywords: biological sciences; cell biology; molecular biology.