Ribosome induces transdifferentiation of A549 and H-111-TC cancer cell lines

Mohammad Badrul Anam; Arif Istiaq; Ryusho Kariya; Mikiko Kudo; Shah Adil Ishtiyaq Ahmad; Naofumi Ito; Seiji Okada; Kunimasa Ohta

doi:10.1016/j.bbrep.2021.100946

Ribosome induces transdifferentiation of A549 and H-111-TC cancer cell lines

Biochem Biophys Rep. 2021 Feb 12:26:100946. doi: 10.1016/j.bbrep.2021.100946. eCollection 2021 Jul.

Authors

Mohammad Badrul Anam^{1

2

3}, Arif Istiaq^{1

4

2

3}, Ryusho Kariya⁵, Mikiko Kudo^{1

4}, Shah Adil Ishtiyaq Ahmad^{1

3

6}, Naofumi Ito^{1

3}, Seiji Okada⁵, Kunimasa Ohta^{1

4

2

3

7}

Affiliations

¹ Department of Developmental Neurobiology, Faculty of Life Sciences, Kumamoto University, Kumamoto, 860-8556, Japan.
² HIGO Program, Kumamoto University, Kumamoto, 860-8556, Japan.
³ Stem Cell-Based Tissue Regeneration Research and Education Unit, Kumamoto University, 1-1-1 Honjo, Chuo-ku, Kumamoto, 860-8556, Japan.
⁴ Department of Stem Cell Biology, Faculty of Arts and Science, Kyushu University, Fukuoka, 819-0395, Japan.
⁵ Division of Hematopoiesis, Joint Research Center for Human Retrovirus Infection, Kumamoto University, Kumamoto, 860-0811, Japan.
⁶ Department of Biotechnology and Genetic Engineering, Mawlana Bhashani Science and Technology University, Tangail, Bangladesh.
⁷ AMED Core Research for Evolutional Science and Technology (AMED-CREST), Japan Agency for Medical Research and Development (AMED), Chiyoda-ku, Tokyo, 100-0004, Japan.

Abstract

Previously we reported that, lactic acid bacteria (LAB) can induce human dermal fibroblast (HDF) cells to form multipotent cell clusters which are able to transdifferentiate into three germ layer derived cell lineages. Later on, we confirmed that ribosome is responsible for the LAB-induced transdifferentiation and ribosomes from diverse organisms can mimic the LAB effect on HDF cells. In our present study we have shown that, upon incorporation of ribosomes, non-small cell lung cancer cell line A549 and gastric tubular adenocarcinoma cell line H-111-TC are transformed into spheroid like morphology those can be transdifferentiated into adipocytes and osteoblast. Our qPCR analysis has revealed that, during the formation of ribosome induced cancer cell spheroids, the expression of the cancer cell associated markers and cell cycle/proliferation markers were altered at different time point. Through our investigation, here we report a novel and a non-invasive approach for cancer cell reprogramming by incorporating ribosomes.

Keywords: Cancer; Cancer cell spheroids; Reprogramming; Ribosome; Transdifferentiation.