Preparation and drying of water-in-oil-in-water (W/O/W) double emulsion to encapsulate soy peptides

Abstract

Soy peptide solution (40%, w/w) was successfully encapsulated in a W₁/O/W₂ double emulsion produced by a two-step emulsification process. Polyglycerol polyricinoleate (PGPR) was found to be an effective inner emulsifier compared to Span 60 and lecithin to produce stable W₁/O primary emulsion. The primary emulsion was subsequently emulsified into an outer aqueous phase (W₂) containing octenyl succinic anhydride (OSA) starch and maltodextrin. The droplet size and encapsulation efficiency of the peptide solution in W₁/O/W₂ emulsion were found to depend on the W₁:O ratio, peptide concentration in the inner W₁ phase and homogenization condition of the secondary emulsification step. The double emulsion with the highest encapsulation efficiency (>80%) was prepared by: (i) using 40% (w/w) soy peptide solution as W₁ phase; (ii) controlling W₁:O ratio at 3:7 (w/w) and (iii) homogenizing the emulsion at 10,000 rpm for 3 min. The freeze-dried microcapsule powder of W₁/O/W₂ emulsion showed higher encapsulation efficiency (>70%) compared to spray-dried one. The freeze-dried microcapsule of W₁/O/W₂ double emulsion developed in this study is a promising delivery matrix to encapsulate hydrophilic ingredients including peptides. Fourier-transform infrared spectroscopy (FTIR) spectra of the microcapsule powder indicated good compatibility between peptide and encapsulants.

Keywords: Double emulsion; Encapsulation; Freeze-drying; Microcapsule; Soy peptides; Spray-drying.