Photoactive and antioxidant nanochitosan dots/biocellulose hydrogels for wound healing treatment

Danica Z Zmejkoski; Zoran M Marković; Milica D Budimir; Nemanja M Zdravković; Dijana D Trišić; Nikol Bugárová; Martin Danko; Natalia O Kozyrovska; Zdeno Špitalský; Angela Kleinová; Sanja B Kuzman; Vladimir B Pavlović; Biljana M Todorović Marković

doi:10.1016/j.msec.2021.111925

Photoactive and antioxidant nanochitosan dots/biocellulose hydrogels for wound healing treatment

Mater Sci Eng C Mater Biol Appl. 2021 Mar:122:111925. doi: 10.1016/j.msec.2021.111925. Epub 2021 Feb 3.

Affiliations

¹ Vinča Institute of Nuclear Sciences - National Institute of the Republic of Serbia, University of Belgrade, P.O.B. 522, 11001, Belgrade, Serbia. Electronic address: danica@vin.bg.ac.rs.
² Vinča Institute of Nuclear Sciences - National Institute of the Republic of Serbia, University of Belgrade, P.O.B. 522, 11001, Belgrade, Serbia. Electronic address: zoranmarkovic@vin.bg.ac.rs.
³ Vinča Institute of Nuclear Sciences - National Institute of the Republic of Serbia, University of Belgrade, P.O.B. 522, 11001, Belgrade, Serbia.
⁴ Scientific Veterinary Institute of Serbia, Janisa Janulisa 14, 11107, Belgrade, Serbia.
⁵ Faculty of Dental Medicine, University of Belgrade, Dr. Subotića 8, 11000, Belgrade, Serbia. Electronic address: dijana.trisic@stomf.bg.ac.rs.
⁶ Polymer Institute, Slovak Academy of Sciences, Dúbravska cestá 9, 84541, Bratislava, Slovakia. Electronic address: nikola.bugarova@savba.sk.
⁷ Polymer Institute, Slovak Academy of Sciences, Dúbravska cestá 9, 84541, Bratislava, Slovakia. Electronic address: upoldan@savba.sk.
⁸ Institute of Molecular Biology and Genetics, National Academy of Sciences of Ukraine, 150, Zabolotnogo Str., Kyiv, 03143, Ukraine.
⁹ Polymer Institute, Slovak Academy of Sciences, Dúbravska cestá 9, 84541, Bratislava, Slovakia. Electronic address: zdeno.spitalsky@savba.sk.
¹⁰ Polymer Institute, Slovak Academy of Sciences, Dúbravska cestá 9, 84541, Bratislava, Slovakia. Electronic address: upolklan@savba.sk.
¹¹ Faculty of Agriculture, University of Belgrade, Nemanjina 6, 11080, Belgrade-Zemun, Serbia. Electronic address: vlaver@agrif.bg.ac.rs.
¹² Vinča Institute of Nuclear Sciences - National Institute of the Republic of Serbia, University of Belgrade, P.O.B. 522, 11001, Belgrade, Serbia. Electronic address: biljatod@vin.bg.ac.rs.

PMID: 33641918
DOI: 10.1016/j.msec.2021.111925

Abstract

Bacterial infection and their resistance to known antibiotics delays wound healing. In this study, nanochitosan dots (nChiD) produced by gamma irradiation have been encapsulated in bacterial cellulose (BC) polymer matrix to study the antibacterial potentials of these nanocomposites and their possible usage in wound healing treatment (scratch assay). Detailed analyses show that nChiDs have disc-like shape and average diameter in the range of 40 to 60 nm depending of the applied dose. All nChiDs as well as BC-nChiD nanocomposites emit green photoluminescence independently on the excitation wavelengths. The new designed nanocomposites do not have a cytotoxic effect; antioxidant analysis shows their moderate radical scavenging activity whereas antibacterial properties show significant growth inhibition of strains mostly found in difficult-to-heal wounds. The obtained results confirm that new designed BC-nChiD nanocomposites might be potential agent in wound healing treatment.

Keywords: Bacterial cellulose; Nanochitosan dots; Nanocomposite; Photoluminescence; Wound healing.

MeSH terms

Anti-Bacterial Agents / pharmacology
Anti-Bacterial Agents / therapeutic use
Antioxidants* / pharmacology
Cellulose
Hydrogels
Nanocomposites*
Wound Healing

Substances

Anti-Bacterial Agents
Antioxidants
Hydrogels
Cellulose