Novel impedimetric sensing strategy for detecting ochratoxin A based on NH2-MIL-101(Fe) metal-organic framework doped with cobalt phthalocyanine nanoparticles

Yingpan Song; Lina He; Shuai Zhang; Xiao Liu; Kun Chen; Qiaojuan Jia; Zhihong Zhang; Miao Du

doi:10.1016/j.foodchem.2021.129248

Novel impedimetric sensing strategy for detecting ochratoxin A based on NH₂-MIL-101(Fe) metal-organic framework doped with cobalt phthalocyanine nanoparticles

Food Chem. 2021 Jul 30:351:129248. doi: 10.1016/j.foodchem.2021.129248. Epub 2021 Feb 18.

Authors

Yingpan Song¹, Lina He², Shuai Zhang², Xiao Liu³, Kun Chen², Qiaojuan Jia², Zhihong Zhang⁴, Miao Du⁵

Affiliations

¹ College of Material and Chemical Engineering, Zhengzhou University of Light Industry, Zhengzhou 450001, PR China; Henan Key Laboratory of Cold Chain Food Quality and Safety Control, Zhengzhou University of Light Industry, Zhengzhou 450001, PR China.
² College of Material and Chemical Engineering, Zhengzhou University of Light Industry, Zhengzhou 450001, PR China.
³ Henan Key Laboratory of Cold Chain Food Quality and Safety Control, Zhengzhou University of Light Industry, Zhengzhou 450001, PR China.
⁴ College of Material and Chemical Engineering, Zhengzhou University of Light Industry, Zhengzhou 450001, PR China. Electronic address: 2006025@zzuli.edu.cn.
⁵ College of Material and Chemical Engineering, Zhengzhou University of Light Industry, Zhengzhou 450001, PR China. Electronic address: dumiao@zzuli.edu.cn.

PMID: 33640766
DOI: 10.1016/j.foodchem.2021.129248

Abstract

Iron-based metal-organic framework, NH₂-MIL-101(Fe), was doped with different dosages of cobalt phthalocyanine nanoparticles (CoPc) to synthesize a series of NH₂-MIL-101(Fe)@CoPc nanocomposites. The NH₂-MIL-101(Fe)@CoPc nanocomposites were then employed to construct novel impedimetric aptasensors for the detection of ochratoxin A (OTA). Combining the intrinsic advantages of NH₂-MIL-101(Fe) (highly porous structure and excellently electrochemical activity) and CoPc (good physiochemical stability and strong bioaffinity), the NH₂-MIL-101(Fe)@CoPc nanocomposites show promising properties, which are beneficial for immobilizing OTA-targeted aptamer strands. Amongst, the developed impedimetric aptasensor based on NH₂-MIL-101(Fe)@CoPc_6:1, prepared using the mass ratio of NH₂-MIL-101(Fe):CoPc of 6:1, exhibits the best amplified electrochemical signal and highest sensitivity for detecting OTA. The detection limitation is 0.063 fg·mL^-1 within the OTA concentration of 0.0001-100 pg·mL^-1, accompanying with high selectivity, good reproducibility and stability, acceptable regenerability, and wide applicability in diverse real samples. Consequently, the proposed sensing strategy can be applied for detecting OTA to cope with food safety.

Keywords: Cobalt phthalocyanine; Detection of ochratoxin A; Food safety; Impedimetric aptasensor; NH(2)-MIL-101(Fe) metal–organic framework.

MeSH terms

Chemistry Techniques, Analytical / instrumentation*
Indoles / chemistry*
Limit of Detection
Metal-Organic Frameworks / chemistry*
Nanoparticles / chemistry*
Ochratoxins / analysis*
Organometallic Compounds / chemistry*
Reproducibility of Results

Substances

Indoles
MIL-101
Metal-Organic Frameworks
Ochratoxins
Organometallic Compounds
ochratoxin A
cobalt phthalocyanine