A novel pilot-scale IFAS-MBR system with low aeration for municipal wastewater treatment: Linkages between nutrient removal and core functional microbiota

Abstract

In this study, we proposed a novel IFAS-MBR with low aeration for the treatment of real municipal wastewater. With biocarriers packed in the anoxic tank, the pilot-scale IFAS-MBR operated with average dissolved oxygen concentrations of 0.56 mg/L in the oxic tank. Over 110 days of operation, highly efficient nutrient removal was achieved with the total nitrogen (TN) and phosphorus (TP) removal efficiencies of 78.1 ± 7.2% and 93.7 ± 5.8%, respectively. The average effluent concentrations of TN and TP reached 5.4 and 0.26 mg/L, respectively. Meanwhile, the removal efficiency of COD reached 95.3 ± 1.3% in the system, and the concentrations of COD decreased from 31.9 ± 3.7 (sludge supernatant) to 12.7 ± 1.6 mg/L (permeate) after membrane filtration. Microbial community analysis showed that Nitrosomonas (0.32%) and Nitrospira (1.85%) in activated sludge were the main drivers of the nitrification process, while various denitrifying bacteria in activated sludge and biofilms were responsible for nitrate reduction in the anoxic tank. Candidatus Accumulibacter (0.34%) and Dechloromonas (1.31%) primarily contributed to denitrifying phosphorus uptake in the anoxic tank. Furthermore, these organisms (i.e., core functional microbiota) exhibited stable levels over the entire operation. The highly enriched hydrolytic fermentation bacteria drove community succession, and the remarkable functional robustness of microbial communities in activated sludge and biofilms favored nutrient removal. Overall, the novel IFAS-MBR system provides an energy-efficient MBR alternative owing to its highly efficient performance and low operating costs enabled by low aeration rates and the absence of an external carbon source.

Keywords: Biofilm; Core functional microbiota; Functional robustness; Low dissolved oxygen.