Analysis of the effects of Photodynamic therapy with Photodithazine on the treatment of 9l/lacZ cells, in vitro study

Gabrielle Dos Santos Vitorio; Rainara Moreno Sanches de Almeida; Juliana Guerra Pinto; Leticia Correa Fontana; Juliana Ferreira-Strixino

doi:10.1016/j.pdpdt.2021.102233

Analysis of the effects of Photodynamic therapy with Photodithazine on the treatment of 9l/lacZ cells, in vitro study

Photodiagnosis Photodyn Ther. 2021 Jun:34:102233. doi: 10.1016/j.pdpdt.2021.102233. Epub 2021 Feb 24.

Authors

Gabrielle Dos Santos Vitorio¹, Rainara Moreno Sanches de Almeida¹, Juliana Guerra Pinto¹, Leticia Correa Fontana¹, Juliana Ferreira-Strixino²

Affiliations

¹ Laboratory of Photobiology Applied to Health, University of Vale do Paraíba/Research and Development Institute, Avenida Shishima Hifumi, 2911, Urbanova, 12244-000, São José dos Campos, SP, Brazil.
² Laboratory of Photobiology Applied to Health, University of Vale do Paraíba/Research and Development Institute, Avenida Shishima Hifumi, 2911, Urbanova, 12244-000, São José dos Campos, SP, Brazil. Electronic address: juferreira@univap.br.

PMID: 33639321
DOI: 10.1016/j.pdpdt.2021.102233

Abstract

Gliosarcoma is an aggressive brain tumor. Photodynamic Therapy (PDT) is a treatment that can be used for various cancers of the CNS. The aim of this study was to analyze the effects of PDT with Photodithazine (PDZ) in the treatment of gliosarcoma, using 9 L/lacZ cells and serial concentrations of 200 μg/mL to 3.1 μg/mL of PDZ. The samples were divided into two groups: dark and light (10 J/cm²). The PDZ was internalized along all the cytoplasmic extension. Viability tests demonstrated a reduction in viable cells after PDT. The production of ROS was concentration-dependent and PDZ was found in mitochondria and lysosomes, presenting a discrete connection with α-tubulin. However, this structure is likely damaged, evidenced by changes in the morphological analysis. Thus, according to the parameters of this study, PDZ proved to be an interesting PS in PDT for the treatment of gliosarcoma, with the inherent limitations of an in vitro study.

Keywords: Brain cancer; Chlorin; Confocal microscopy; Photodynamic therapy.

MeSH terms

Cell Line, Tumor
Gliosarcoma* / drug therapy
Glucosamine / analogs & derivatives
Humans
Lac Operon
Photochemotherapy* / methods
Photosensitizing Agents / pharmacology
Photosensitizing Agents / therapeutic use

Substances

Photosensitizing Agents
N-methylglucosamine
Glucosamine