A High-Efficiency Artificial Synthetic Pathway for 5-Aminovalerate Production From Biobased L-Lysine in Escherichia coli

Jie Cheng; Wenying Tu; Zhou Luo; Xinghua Gou; Qiang Li; Dan Wang; Jingwen Zhou

doi:10.3389/fbioe.2021.633028

A High-Efficiency Artificial Synthetic Pathway for 5-Aminovalerate Production From Biobased L-Lysine in Escherichia coli

Front Bioeng Biotechnol. 2021 Feb 9:9:633028. doi: 10.3389/fbioe.2021.633028. eCollection 2021.

Authors

Jie Cheng¹, Wenying Tu¹, Zhou Luo¹, Xinghua Gou¹, Qiang Li¹, Dan Wang², Jingwen Zhou³

Affiliations

¹ Key Laboratory of Meat Processing of Sichuan Province, Key Laboratory of Coarse Cereal Processing, Ministry of Agriculture and Rural Affairs, College of Food and Biological Engineering, Chengdu University, Chengdu, China.
² Department of Chemical Engineering, School of Chemistry and Chemical Engineering, Chongqing University, Chongqing, China.
³ National Engineering Laboratory for Cereal Fermentation Technology, Jiangnan University, Wuxi, China.

Abstract

Bioproduction of 5-aminovalerate (5AVA) from renewable feedstock can support a sustainable biorefinery process to produce bioplastics, such as nylon 5 and nylon 56. In order to achieve the biobased production of 5AVA, a 2-keto-6-aminocaproate-mediated synthetic pathway was established. Combination of L-Lysine α-oxidase from Scomber japonicus, α-ketoacid decarboxylase from Lactococcus lactis and aldehyde dehydrogenase from Escherichia coli could achieve the biosynthesis of 5AVA from biobased L-Lysine in E. coli. The H₂O₂ produced by L-Lysine α-oxidase was decomposed by the expression of catalase KatE. Finally, 52.24 g/L of 5AVA were obtained through fed-batch biotransformation. Moreover, homology modeling, molecular docking and molecular dynamic simulation analyses were used to identify mutation sites and propose a possible trait-improvement strategy: the expanded catalytic channel of mutant and more hydrogen bonds formed might be beneficial for the substrates stretch. In summary, we have developed a promising artificial pathway for efficient 5AVA synthesis.

Keywords: 5-aminovalerate; L-Lysine HCl; artificial pathway; molecular docking; molecular dynamic simulation.