Directed Evolution of Propionyl-CoA Carboxylase for Succinate Biosynthesis

Yuwan Liu; Huifeng Jiang

doi:10.1016/j.tibtech.2021.02.006

Directed Evolution of Propionyl-CoA Carboxylase for Succinate Biosynthesis

Trends Biotechnol. 2021 Apr;39(4):330-331. doi: 10.1016/j.tibtech.2021.02.006. Epub 2021 Feb 22.

Authors

Yuwan Liu¹, Huifeng Jiang²

Affiliations

¹ Key Laboratory of Systems Microbial Biotechnology, Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences, Tianjin, 300308, China; National Technology Innovation Center of Synthetic Biology, Tianjin, 300308, China.
² Key Laboratory of Systems Microbial Biotechnology, Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences, Tianjin, 300308, China; National Technology Innovation Center of Synthetic Biology, Tianjin, 300308, China. Electronic address: jiang_hf@tib.cas.cn.

PMID: 33632542
DOI: 10.1016/j.tibtech.2021.02.006

Abstract

Due to low carboxylase activity, CO₂ biotransformation is challenging to achieve using natural CO₂ fixation pathways. Liu et al. have improved the activity of propionyl-CoA carboxylase (PCC) 94-fold, enabling the efficient synthesis of succinate from acetyl-CoA and paving the way for CO₂ assimilation via the 3-hydroxypropionate (3-HP) bicycle or 3-hydroxypropionate/4-hydroxybutyrate (3-HP/4-HB) cycle.

Publication types

Review

MeSH terms

Biotechnology* / methods
Carbon Dioxide / metabolism
Directed Molecular Evolution*
Methylmalonyl-CoA Decarboxylase / genetics
Succinates / metabolism
Succinic Acid* / metabolism

Substances

Succinates
Carbon Dioxide
Succinic Acid
Methylmalonyl-CoA Decarboxylase