Ozonated Aloe vera Oil Effective Increased the Number of Fibroblasts and Collagen Thickening in the Healing Response of Full-Thickness Skin Defects

Ahsanu Taqwim Hidayat; Muhamad Thohar Arifin; Muhammad Nur; Muflihatul Muniroh; Neni Susilaningsih

doi:10.1155/2021/6654343

Ozonated Aloe vera Oil Effective Increased the Number of Fibroblasts and Collagen Thickening in the Healing Response of Full-Thickness Skin Defects

Int J Inflam. 2021 Feb 9:2021:6654343. doi: 10.1155/2021/6654343. eCollection 2021.

Authors

Ahsanu Taqwim Hidayat¹, Muhamad Thohar Arifin^{1

2}, Muhammad Nur³, Muflihatul Muniroh^{1

4}, Neni Susilaningsih^{1

5}

Affiliations

¹ Master Student of Biomedical Sciences, Faculty of Medicine, Diponegoro University, Semarang, Indonesia.
² Department of Neurosurgery, Faculty of Medicine, Diponegoro University, Semarang, Indonesia.
³ Division of Physics, Faculty of Science and Mathematics, Diponegoro University, Semarang, Indonesia.
⁴ Department of Physiology, Faculty of Medicine, Diponegoro University, Semarang, Indonesia.
⁵ Department of Histology, Faculty of Medicine, Diponegoro University, Semarang, Indonesia.

Abstract

Objective: This study aimed to examine the effectiveness of ozonated Aloe vera oil on the wound healing response of full-thickness defect tissue in Sprague-Dawley rats, assessed by collagen thickness and the number of fibroblasts.

Methods: This was an experimental research method using control groups and treatment groups with a posttest only control group design. The results showed that collagen thickness in wounds tended to increase, assessed on day 3 and day 7 using Masson's trichrome staining and microscopic evaluation.

Results: There was a significant difference in the number of fibroblasts between the two control and treatment groups on days 3 and 7 tested using one-way Kruskal-Wallis test, with a value of p=0.001(p < 0.05), resulting in a significant difference in wound size reduction between the groups. Further post hoc analysis using the Mann-Whitney test indicated a significant difference between the control groups and the treatment groups (P0, P1 versus P3, P4, P5, P8, P9, and P10) with a value of p=0.009(p < 0.05).

Conclusions: Ozonated Aloe vera oil is effective in increasing the healing response of full-thickness defects, leading to the increase in the number of fibroblasts and collagen thickening that in turn accelerates wound healing in Sprague-Dawley rats.