Comparative and Combined In Vitro Vasotoxicity of Nanoparticles Containing Lead and Cadmium

Tatiana V Bushueva; Ilzira A Minigalieva; Vladimir G Panov; Marina P Sutunkova; Vladimir B Gurvich; Vladimir Ya Shur; Ekaterina V Shishkina; Anna S Naumova; Elizaveta P Artemenko; Boris A Katsnelson

doi:10.1177/1559325820982163

Comparative and Combined In Vitro Vasotoxicity of Nanoparticles Containing Lead and Cadmium

Dose Response. 2021 Feb 11;19(1):1559325820982163. doi: 10.1177/1559325820982163. eCollection 2021 Jan-Mar.

Affiliations

¹ The Yekaterinburg Medical Research Center for Prophylaxis and Health Protection in Industrial Workers, Yekaterinburg, Russia.
² Institute of Industrial Ecology, the Urals Branch of the Russian Academy of Sciences, Ekaterinburg, Russia.
³ Institute of Natural Sciences and Mathematics, The Ural Federal University, Yekaterinburg, Russia.

Abstract

In vitro toxicological experiments were performed on an endothelial cell line exposed to different doses of spherical nanoparticles of cadmium and/or of lead sulfides with mean diameter 37 ± 5 nm and 24 ± 4 nm, respectively. Toxic effects were estimated by Luminescent Cell Viability Assay, endothelin-1 concentration and cell size determination. Some dose-response relationships were typically monotonic (well approximated with hyperbolic function) while others were bi- or even 3-phasic and could be described within the expanded hormesis paradigm. The combined toxicity type variated depending on the effect it was assessed by.

Keywords: cadmium; cell cultures; combined toxic action; lead; nanoparticles.