Molecular characterization of clinical carbapenem-resistant Enterobacterales from Qatar

Eur J Clin Microbiol Infect Dis. 2021 Aug;40(8):1779-1785. doi: 10.1007/s10096-021-04185-7. Epub 2021 Feb 22.

Abstract

One hundred forty-nine carbapenem-resistant Enterobacterales from clinical samples obtained between April 2014 and November 2017 were subjected to whole genome sequencing and multi-locus sequence typing. Klebsiella pneumoniae (81, 54.4%) and Escherichia coli (38, 25.5%) were the most common species. Genes encoding metallo-β-lactamases were detected in 68 (45.8%) isolates, and OXA-48-like enzymes in 60 (40.3%). blaNDM-1 (45; 30.2%) and blaOXA-48 (29; 19.5%) were the most frequent. KPC-encoding genes were identified in 5 (3.6%) isolates. Most common sequence types were E. coli ST410 (8; 21.1%) and ST38 (7; 18.4%), and K. pneumoniae ST147 (13; 16%) and ST231 (7; 8.6%).

Keywords: CRE; Carbapenemase; Enterobacterales; KPC; Middle East; NDM; OXA; Qatar; VIM.

MeSH terms

  • Adolescent
  • Adult
  • Aged
  • Aged, 80 and over
  • Anti-Bacterial Agents / pharmacology*
  • Child
  • Child, Preschool
  • Drug Resistance, Multiple, Bacterial*
  • Enterobacteriaceae / drug effects*
  • Enterobacteriaceae / genetics
  • Enterobacteriaceae Infections / drug therapy
  • Enterobacteriaceae Infections / epidemiology
  • Enterobacteriaceae Infections / microbiology*
  • Female
  • Humans
  • Infant
  • Male
  • Middle Aged
  • Qatar / epidemiology
  • Young Adult

Substances

  • Anti-Bacterial Agents