Blood Flow Restriction Training Using the Delfi System Is Associated With a Cellular Systemic Response

Mark C Callanan; Hillary A Plummer; Garrett L Chapman; Tyler J Opitz; Nicole K Rendos; Adam W Anz

doi:10.1016/j.asmr.2020.09.009

Blood Flow Restriction Training Using the Delfi System Is Associated With a Cellular Systemic Response

Arthrosc Sports Med Rehabil. 2020 Dec 27;3(1):e189-e198. doi: 10.1016/j.asmr.2020.09.009. eCollection 2021 Feb.

Authors

Mark C Callanan¹, Hillary A Plummer², Garrett L Chapman³, Tyler J Opitz⁴, Nicole K Rendos², Adam W Anz⁴

Affiliations

¹ The Orthopedic Clinic, Shreveport, Louisiana, U.S.A.
² Andrews Research & Education Foundation, Gulf Breeze, Florida, U.S.A.
³ Beaver Medical Group, Redlands, California, U.S.A.
⁴ Andrews Institute for Orthopedics & Sports Medicine, Gulf Breeze, Florida, U.S.A.

Abstract

Purpose: To determine the effects of blood flow restriction (BFR) exercise on CD34⁺ cells, platelets, white blood cells, neutrophils, lymphocytes, lactate, and glucose.

Methods: Healthy participants aged 20 to 39 years who were able to perform the exercise sessions were recruited. Participants underwent an experimental (EXP) occluded testing session and a control (CON) session using the Delfi Personalized Tourniquet System. Blood draws were performed prior to testing and immediately after the exercise session. Blood analysis consisted of a complete blood count as well as flow cytometry to measure peripheral CD34⁺ counts as a marker for hematopoietic progenitor cells.

Results: Fourteen men (aged 30.8 ± 3.9 years) volunteered. There was a significant increase in average CD34⁺ counts immediately after the EXP session only (3.1 ± 1.2 cells ⋅ μL^-1 vs 5.2 ± 2.9 cells ⋅ μL^-1, P = .012). Platelet counts were significantly elevated after both sessions, with the average increase being higher after the EXP session (mean difference [MD], 34,200/μL; P < .002) than after the CON session (MD, 11,600/μL; P < .002). White blood cell counts significantly increased after both the EXP (8,400 ± 2,200/μL vs 6,300 ± 1,600/μL; P < .001) and CON (MD, 900/μL; P < .001) sessions. There was a significant increase from baseline to immediately after exercise in the average number of lymphocytes (MD, 6.3%; P < .001) and, conversely, a significant decrease in the average neutrophil count (MD, 6.5%; P < .001) in the EXP session only. Lactate levels significantly increased in the EXP (MD, 6.1 mmol ⋅ L^-1; P = .001) and CON (MD, 3.6 mmol ⋅ L^-1; P = .001) groups. No changes in glucose levels were observed.

Conclusions: Exercise with BFR causes a significant post-exercise increase in peripheral hematopoietic progenitor cells and platelets, beyond that of standard resistance training.

Clinical relevance: BFR can be considered a way to manipulate point-of-care blood products such as platelet-rich plasma to increase product yield.