Plastome of Saraca asoca (Detarioideae, Fabaceae): Annotation, comparison among subfamily and molecular typing

Mohammad Ajmal Ali; Tapan Kumar Pan; Arun Bahadur Gurung; Mohammad Abul Farah; Fahad Al-Hemaid; Khalid Mashay Alanazi; Meena Elangbam; Joongku Lee; Shankar Kumar Pandey; M Oliur Rahman; Soo-Yong Kim

doi:10.1016/j.sjbs.2020.12.008

Plastome of Saraca asoca (Detarioideae, Fabaceae): Annotation, comparison among subfamily and molecular typing

Saudi J Biol Sci. 2021 Feb;28(2):1487-1493. doi: 10.1016/j.sjbs.2020.12.008. Epub 2020 Dec 11.

Affiliations

¹ Department of Botany and Microbiology, College of Science, King Saud University, Riyadh 11451, Saudi Arabia.
² University Department of Botany, Tilka Manjhi Bhagalpur University, Bhagalpur 812007, Bihar, India.
³ Department of Basic Sciences and Social Sciences, North-Eastern Hill University, Shillong 793022, Meghalaya, India.
⁴ Genetics Laboratory, Department of Zoology, College of Science, King Saud University, Riyadh 11451, Saudi Arabia.
⁵ Genetics Laboratory, Centre of Advanced Studies in Life Sciences, Manipur University, Canchipur 795 003, India.
⁶ Department of Environment and Forest Resources, Chungnam National University, Daejeon 34134, Republic of Korea.
⁷ Department of Botany, TNB College, Tilka Manjhi Bhagalpur University, Bhagalpur 812007, Bihar, India.
⁸ Department of Botany, University of Dhaka, Dhaka 1000, Bangladesh.
⁹ International Biological Material Research Center, Korea Research Institute of Bioscience and Biotechnology, Daejeon 34141, Republic of Korea.

Abstract

Saraca asoca (Roxb.) Willd. (subfamily Detarioideae, family Fabaceae) is a perennial evergreen sacred medicinal tree classified under 'vulnerable' by the IUCN. The chloroplast (cp) genome/plastome which follows uniparental inheritance contains many useful genetic information because of its conservative rate of evolution. The assembled cp genome of S. asoca which maps as a conserved circular structure revealed extensive rearrangement in gene organization, comprising total length 160,003 bp including LSC, SSC, IRa, and IRb, and GC content was 35.26%. Herein a set of rbcL and matK gene were established using molecular phylogenetic analyses for molecular typing of S. asoca.

Keywords: Detarioideae; Fabaceae; Genomic rearrangement; Molecular authentication; Plastome; Saraca asoca; Simple sequence repeat.