Abstract
A resazurin micro-assay was developed to quantify acidifying bacteria. The resorufin fluorescent signal was measured over time and the determined time to reach the max slope (TMS) was plotted against CFU (colony forming unit) counts. This dynamic assay enabled to quantify nine lactic acid bacteria and a Bacillus licheniformis strain despite the increasing acidity of the medium.
Keywords:
Fluorescence; Lactic acid bacteria; Resazurin microassay; Viability quantification.
Copyright © 2021 Elsevier B.V. All rights reserved.
Publication types
-
Evaluation Study
-
Research Support, Non-U.S. Gov't
MeSH terms
-
Acids / metabolism*
-
Bacillus licheniformis / chemistry
-
Bacillus licheniformis / growth & development
-
Bacillus licheniformis / metabolism
-
Bacteriological Techniques / methods*
-
Culture Media / chemistry
-
Culture Media / metabolism
-
Fluorescence
-
Lactobacillales / chemistry
-
Lactobacillales / growth & development*
-
Lactobacillales / metabolism
-
Oxazines / chemistry*
-
Oxazines / metabolism
-
Xanthenes / chemistry*
-
Xanthenes / metabolism
Substances
-
Acids
-
Culture Media
-
Oxazines
-
Xanthenes
-
resazurin