CRISPR base editor screens identify variant function at scale

Phoebe C R Parrish; Alice H Berger

doi:10.1016/j.molcel.2021.01.036

CRISPR base editor screens identify variant function at scale

Mol Cell. 2021 Feb 18;81(4):647-648. doi: 10.1016/j.molcel.2021.01.036.

Authors

Phoebe C R Parrish¹, Alice H Berger²

Affiliations

¹ Human Biology Division, Fred Hutchinson Cancer Research Center, Seattle, WA, USA; Computational Biology Program, Public Health Sciences Division, Fred Hutchinson Cancer Research Center, Seattle, WA, USA; Department of Genome Sciences, University of Washington, Seattle, WA, USA.
² Human Biology Division, Fred Hutchinson Cancer Research Center, Seattle, WA, USA; Computational Biology Program, Public Health Sciences Division, Fred Hutchinson Cancer Research Center, Seattle, WA, USA; Department of Genome Sciences, University of Washington, Seattle, WA, USA. Electronic address: ahberger@fredhutch.org.

Abstract

Cuella-Martin et al. (2021) and Hanna et al. (2021) showcase CRISPR base editing in large-scale pooled screens in human cells to discover both loss- and gain-of-function variants, enabling protein structure/function insights and clinical variant interpretation.

Publication types

Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, Non-P.H.S.
Comment

MeSH terms

CRISPR-Cas Systems
Clustered Regularly Interspaced Short Palindromic Repeats* / genetics
Gene Editing*
Humans

Grants and funding

P50 CA228944/CA/NCI NIH HHS/United States