Transcriptomic analysis elucidates the molecular processes associated with hydrogen peroxide-induced diapause termination in Artemia-encysted embryos

Bonien Chen; Tah-Wei Chu; Kuohsun Chiu; Ming-Chang Hong; Tsung-Meng Wu; Jui-Wen Ma; Chih-Ming Liang; Wei-Kuang Wang

doi:10.1371/journal.pone.0247160

Transcriptomic analysis elucidates the molecular processes associated with hydrogen peroxide-induced diapause termination in Artemia-encysted embryos

PLoS One. 2021 Feb 19;16(2):e0247160. doi: 10.1371/journal.pone.0247160. eCollection 2021.

Authors

Bonien Chen¹, Tah-Wei Chu¹, Kuohsun Chiu^{1

2}, Ming-Chang Hong¹, Tsung-Meng Wu³, Jui-Wen Ma¹, Chih-Ming Liang⁴, Wei-Kuang Wang⁴

Affiliations

¹ Department and Graduate Institute of Aquaculture, National Kaohsiung University of Science and Technology, Kaohsiung, Taiwan.
² Department of Oceanography, National Sun Yat-sen University, Kaohsiung, Taiwan.
³ Department of Aquaculture, National Pingtung University of Science and Technology, Pingtung, Taiwan.
⁴ Department of Environmental Engineering and Science, Feng Chia University, Taichung, Taiwan.

Abstract

Treatment with hydrogen peroxide (H2O2) raises the hatching rate through the development and diapause termination of Artemia cysts. To comprehend the upstream genetic regulation of diapause termination activated by exterior H2O2 elements, an Illumina RNA-seq analysis was performed to recognize and assess comparative transcript amounts to explore the genetic regulation of H2O2 in starting the diapause termination of cysts in Artemia salina. We examined three groupings treated with no H2O2 (control), 180 μM H2O2 (low) and 1800 μM H2O2 (high). The results showed a total of 114,057 unigenes were identified, 41.22% of which were functionally annotated in at least one particular database. When compared to control group, 34 and 98 differentially expressed genes (DEGs) were upregulated in 180 μM and 1800 μM H2O2 treatments, respectively. On the other hand, 162 and 30 DEGs were downregulated in the 180 μM and 1800 μM H2O2 treatments, respectively. Cluster analysis of DEGs demonstrated significant patterns among these types of 3 groups. GO and KEGG enrichment analysis showed the DEGs involved in the regulation of blood coagulation (GO: 0030193; GO: 0050818), regulation of wound healing (GO:0061041), regulation of hemostasis (GO: 1900046), antigen processing and presentation (KO04612), the Hippo signaling pathway (KO04391), as well as the MAPK signaling pathway (KO04010). This research helped to define the diapause-related transcriptomes of Artemia cysts using RNA-seq technology, which might fill up a gap in the prevailing body of knowledge.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Artemia / drug effects
Artemia / genetics*
Diapause / drug effects
Diapause / genetics*
Down-Regulation / drug effects
Embryo, Nonmammalian / drug effects
Embryo, Nonmammalian / metabolism*
Gene Expression Profiling*
Gene Expression Regulation, Developmental / drug effects
Gene Ontology
Hydrogen Peroxide / toxicity*
Molecular Sequence Annotation
Up-Regulation / drug effects
Up-Regulation / genetics

Substances

Hydrogen Peroxide

Grants and funding

This work partially supported by Ministry of Science and Technology, Taiwan (MOST 108-2221-E-035-057 and MOST 109-2221-E-035-034). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.