Computer-Assisted Sperm Analysis to Test Environmental Toxicants

Tímea Kollár; Ákos Horváth; Zsolt Csenki-Bakos

doi:10.1007/978-1-0716-0970-5_3

Computer-Assisted Sperm Analysis to Test Environmental Toxicants

Methods Mol Biol. 2021:2218:29-35. doi: 10.1007/978-1-0716-0970-5_3.

Authors

Tímea Kollár¹, Ákos Horváth², Zsolt Csenki-Bakos¹

Affiliations

¹ Department of Aquaculture, Szent István University, Gödöllő, Páter Károly u. 1., Hungary.
² Department of Aquaculture, Szent István University, Gödöllő, Páter Károly u. 1., Hungary. Horvath.Akos@szie.hu.

PMID: 33606220
DOI: 10.1007/978-1-0716-0970-5_3

Abstract

Fish sperm show many measurable parameters which react sensitively in a dose- and time-dependent way to toxic exposure. Fish sperm is therefore used as an in vitro toxicology test system. One of the most sensitive and easily detectable parameters is progressive motility which can be measured by a computer-assisted sperm analysis (CASA) system. Here we describe a simple protocol to test the effect of environmental toxicants by using zebrafish (Danio rerio) sperm.

Keywords: CASA; Progressive motility; Zebrafish sperm.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Computers
Hazardous Substances / toxicity*
Image Processing, Computer-Assisted / methods*
Male
Semen Analysis / methods*
Sperm Motility / drug effects
Sperm Motility / physiology
Spermatozoa / drug effects
Spermatozoa / physiology*
Zebrafish / physiology*

Substances

Hazardous Substances