Monitoring daytime differences in moderate intensity exercise capacity using treadmill test and muscle dissection

Yaarit Adamovich; Saar Ezagouri; Vaishnavi Dandavate; Gad Asher

doi:10.1016/j.xpro.2021.100331

Monitoring daytime differences in moderate intensity exercise capacity using treadmill test and muscle dissection

STAR Protoc. 2021 Feb 5;2(1):100331. doi: 10.1016/j.xpro.2021.100331. eCollection 2021 Mar 19.

Authors

Yaarit Adamovich¹, Saar Ezagouri¹, Vaishnavi Dandavate¹, Gad Asher¹

Affiliation

¹ Department of Biomolecular Sciences, Weizmann Institute of Science, 7610001 Rehovot, Israel.

Abstract

There is growing interest in medicine and sports in uncovering exercise modifiers that enhance or limit exercise capacity. Here, we detail a protocol for testing the daytime effect on running capacity in mice using a moderate intensity treadmill effort test. Instructions for dissecting soleus, gastrocnemius plantaris, and quadriceps muscles for further analysis are provided as well. This experimental setup is optimized for addressing questions regarding the involvement of daytime and circadian clocks in regulating exercise capacity. For complete details on the use and execution of this protocol, please refer to Ezagouri et al. (2019).

Keywords: Metabolism; Model organisms.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Exercise Tolerance*
Male
Mice
Microdissection*
Muscle, Skeletal*
Physical Conditioning, Animal*