Using high-speed videography for objective and reproducible pain measurement on a mouse pain scale

Justin Burdge; Nathan T Fried; Ishmail Abdus-Saboor

doi:10.1016/j.xpro.2021.100322

Using high-speed videography for objective and reproducible pain measurement on a mouse pain scale

STAR Protoc. 2021 Feb 5;2(1):100322. doi: 10.1016/j.xpro.2021.100322. eCollection 2021 Mar 19.

Authors

Justin Burdge¹, Nathan T Fried², Ishmail Abdus-Saboor¹

Affiliations

¹ University of Pennsylvania, Department of Biology, Philadelphia, PA 19104, USA.
² Rutgers University, Department of Biology, Camden, NJ 08102, USA.

Abstract

Mouse models are essential for studying pain neurobiology and testing pain therapeutics. The reliance on assays that only measure the presence, absence, or frequency of a reflex have limited the reliability of preclinical pain studies. Our high-speed videography protocol overcomes this by projecting the discrete sub-second kinematic behavioral features induced by hind paw stimulation onto a "mouse pain scale." This provides a more objective and robust pain measurement in mice by quantifying the quality of the stimulus-induced hind paw reflex. For complete details on the use and execution of this protocol, please refer to Abdus-Saboor et al. (2019).

Keywords: Model organisms; Neuroscience.

Publication types

Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't

MeSH terms

Animals
Disease Models, Animal
Mice
Pain / physiopathology*
Pain Measurement*

Grants and funding

R00 DE026807/DE/NIDCR NIH HHS/United States